The simultaneous assay of emetine and cephaeline in ipecacuanha and its preparations by spectrofluorimetry.

The simultaneous assay of emetine and cephaeline in ipecacuanha and its preparations by spectrofluorimetry. Research Abstract Details 

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The simultaneous assay of emetine and cephaeline in ipecacuanha and its preparations by spectrofluorimetry. Abstract Text:

A G Davidson,S M Hassan,

A rapid assay is described for the simultaneous determination of emetine and cephaeline in ipecacuanha and its preparations, based upon the different fluorescence intensities of the alkaloids at pH 1 and pH 12. The assay involves the measurement of fluorescence at 317 nm of dilutions of the sample in 0.1 M hydrochloric acid (pH 1) and 0.01 M sodium hydroxide (pH 12) with an excitation wavelength of 283 nm. Concentrations of the individual alkaloids are calculated using two simultaneous equations derived from the experimentally determined coefficients of fluorescence of solutions (1 mug/ml) of emetine and cephaeline at pH 1 and pH 12. The procedure, which has been shown to be accurate, precise and sensitive, requires only 1 ml of a liquid sample and less than 0.5 g of powdered root. There was reasonable agreement between the total concentrations of alkaloids in tinctures and liquid extracts of ipecacuanha determined by the spectrofluorimetric method and by the titrimetric procedure of the British Pharmacopoeia. The fluorimetric procedure gave higher levels of alkaloids in the powdered root than did the B.P. method; this difference is explained by incomplete extraction of the alkaloids in the B.P. procedure for powdered ipecacuanha root.

The simultaneous assay of emetine and cephaeline in ipecacuanha and its preparations by spectrofluorimetry. Publishing Authors By Initials

AG Davidson,SM Hassan,

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The simultaneous assay of emetine and cephaeline in ipecacuanha and its preparations by spectrofluorimetry. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of pharmaceutical and biomedical analysis

VOLUME: 2

Page Numbers: 441-8

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ISSN: 0731-7085

DAY: 26

MONTH: 07

YEAR: 1984

The simultaneous assay of emetine and cephaeline in ipecacuanha and its preparations by spectrofluorimetry. Information

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LANGUAGE: eng

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Grant and Affiliation Information for The simultaneous assay of emetine and cephaeline in ipecacuanha and its preparations by spectrofluorimetry.

AFFILIATION: Department of Pharmacy, University of Strathclyde, George Street, Glasgow G1 1XW, UK.

Country: England

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MEDLINETA: J Pharm Biomed Anal

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