The separation of neutral glycosphingolipids from mammalian erythrocytes by droplet counter-current chromatography (DCC).

The separation of neutral glycosphingolipids from mammalian erythrocytes by droplet counter-current chromatography (DCC). Research Abstract Details 

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The separation of neutral glycosphingolipids from mammalian erythrocytes by droplet counter-current chromatography (DCC). Abstract Text:

H Otsuka,A Suzuki,T Yamakawa,

In a previous paper (Otsuka, H. & Yamakawa, T. (1981) J. Biochem. 90, 247-254), we reported the separation of acidic glycolipids by droplet counter-current chromatography using 500 columns with a commercially available DCC apparatus and described the precise conditions of the separation. In this paper, separation of neutral glycolipids from rabbit and human erythrocytes by DCC is described. For efficient separation of neutral glycolipids, addition of a definite amount of benzene was required. The solvent system of chloroform: benzene: methanol: water = 50: 50: 70: 20 gave clear separation of these glycolipids and further modification of this solvent system to chloroform: benzene: methanol: water = 50: 25: 65: 30 gave satisfactory results for the separation of Globoside I from human erythrocytes due to the difference between the amide linked fatty acids. Also under the same conditions, the clear separation of two peaks with blood group A activity was demonstrated by a hemagglutination inhibition test.

The separation of neutral glycosphingolipids from mammalian erythrocytes by droplet counter-current chromatography (DCC). Publishing Authors By Initials

H Otsuka,A Suzuki,T Yamakawa,

For similar biological phenomena, cell phenomena, and immunity: immunity: antibody specificity: species specificity research abstracts see: biological phenomena, cell phenomena, and immunity: immunity: antibody specificity: species specificity research

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The separation of neutral glycosphingolipids from mammalian erythrocytes by droplet counter-current chromatography (DCC). Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 94

Page Numbers: 2035-41

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Dec

YEAR: 1983

The separation of neutral glycosphingolipids from mammalian erythrocytes by droplet counter-current chromatography (DCC). Information

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LANGUAGE: eng

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The separation of neutral glycosphingolipids from mammalian erythrocytes by droplet counter-current chromatography (DCC). Keywords Mesh Terms:

KEYWORDS: Species Specificity

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: The separation of neutral glycosphingolipids from mammalian erythrocytes by droplet counter-current chromatography (DCC). Information

Substance Name: Glycosphingolipids

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Country: JAPAN

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MEDLINETA: J Biochem

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