The self-assembly of biodegradable cationic polymer micelles as vectors for gene transfection.

The self-assembly of biodegradable cationic polymer micelles as vectors for gene transfection. Research Abstract Details 

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The self-assembly of biodegradable cationic polymer micelles as vectors for gene transfection. Abstract Text:

Yong Wang,Chyan-Ying Ke,Cyrus Weijie Beh,Shao-Qiong Liu,Suat-Hong Goh,Yi-Yan Yang,Yong Wang,Chyan-Ying Ke,Cyrus Weijie Beh,Shao-Qiong Liu,Suat-Hong Goh,Yi-Yan Yang,Yong Wang,Chyan-Ying Ke,Cyrus Weijie Beh,Shao-Qiong Liu,Suat-Hong Goh,Yi-Yan Yang,

Cationic micelles self-assembled from a biodegradable amphiphilic copolymer, poly{(N-methyldietheneamine sebacate)-co-[(cholesteryl oxocarbonylamido ethyl) methyl bis(ethylene) ammonium bromide] sebacate} (P(MDS-co-CES)) have recently been reported for efficient gene delivery and co-delivery of drug and nucleic acid. In this study, poly(ethylene glycol) (PEG) of various molecular weights (M(n)=550, 1100 and 2000) was conjugated to P(MDS-co-CES) having different cholesterol grafting degrees to improve the stability of micelle/DNA complexes in the blood for systemic in vivo gene delivery. DNA binding ability, gene transfection efficiency and cytotoxicity of P(MDS-co-CES), PMDS, PEGylated PMDS and PEGylated P(MDS-co-CES) micelles were studied and compared. As with P(MDS-co-CES), PEG-P(MDS-co-CES) polymers could also self-assemble into stable micelles of small size. However, PMDS and PEG-PMDS without cholesterol could not form stable micelles but formed large particles. PEGylation of polymers significantly decreased their gene transfection efficiency in HEK293, HepG2, HeLa, MDA-MB-231 and 4T1 cells. However, increasing N/P ratio promoted gene transfection. An increased cholesterol grafting degree led to greater gene expression level possibly because of the more stable core-shell structure of the micelles. PEG550-P(MDS-co-CES) micelles induced high gene transfection level, comparable to that provided by P(MDS-co-CES) micelles. PEGylated polymers were much less cytotoxic than P(MDS-co-CES). PEGylated P(MDS-co-CES) micelles may provide a promising non-viral vector for systemic in vivo gene delivery.

The self-assembly of biodegradable cationic polymer micelles as vectors for gene transfection. Publishing Authors By Initials

Y Wang,CY Ke,C Weijie Beh,SQ Liu,SH Goh,YY Yang,Y Wang,CY Ke,C Weijie Beh,SQ Liu,SH Goh,YY Yang,Y Wang,CY Ke,C Weijie Beh,SQ Liu,SH Goh,YY Yang,

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The self-assembly of biodegradable cationic polymer micelles as vectors for gene transfection. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biomaterials

VOLUME: 28

Page Numbers: 5358-68

Journal Abbreviation: Biomaterials

ISSN: 0142-9612

DAY: 30

MONTH: 08

YEAR: 2007

The self-assembly of biodegradable cationic polymer micelles as vectors for gene transfection. Information

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LANGUAGE: eng

NlmUniqueID: 8100316

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Grant and Affiliation Information for The self-assembly of biodegradable cationic polymer micelles as vectors for gene transfection.

AFFILIATION: Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, Singapore 138669, Singapore; Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore.

Country: England

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MEDLINETA: Biomaterials

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