The salivary mucin MG1 (MUC5B) carries a repertoire of unique oligosaccharides that is large and diverse.

The salivary mucin MG1 (MUC5B) carries a repertoire of unique oligosaccharides that is large and diverse. Research Abstract Details 

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The salivary mucin MG1 (MUC5B) carries a repertoire of unique oligosaccharides that is large and diverse. Abstract Text:

Kristina A Thomsson,Akraporn Prakobphol,Hakon Leffler,Molakala S Reddy,Michael J Levine,Susan J Fisher,Gunnar C Hansson,

The high-molecular-mass salivary mucin MG1, one of two major mucins produced by human salivary glands, plays an important role in oral health by coating the tooth surface and by acting as a bacterial receptor. Here this mucin was purified from the submandibular/sublingual saliva of a blood group O individual. The presence of MUC5B as the major mucin in this preparation was confirmed by amino acid analysis and its reactivity with the monoclonal antibody PAN H2. To structurally characterize MG1 carbohydrates the O-glycans were released by reductive beta-elimination. Nuclear magnetic resonance spectroscopy of the nonfractionated mixture showed that (1) fucose was present in blood group H, Le(a), Le(x), Le(b), and Le(y) epitopes; (2) NeuAc was mainly linked alpha 2-3 to Gal or alpha 2-6 to GalNAcol; and (3) the major internal structures were core 1 and core 2 sequences. After this preliminary analysis the released oligosaccharides were separated into neutral (56%), sialylated (26%), and sulfated (19%) fractions, with an average length of 13, 17, and 41 sugar residues, respectively. Gas chromatography-mass spectrometry and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of mixtures of neutral and sialylated oligosaccharides revealed at least 62 neutral and 25 sialylated oligosaccharides consisting of up to 20 monosaccharide residues. These results showed that the MG1-derived oligosaccharides were much longer than those of MG2, and only a few species were found on both molecules. Thus, these two mucins create an enormous repertoire of potential binding sites for microorganisms at one of the major portals where infectious organisms enter the body.

The salivary mucin MG1 (MUC5B) carries a repertoire of unique oligosaccharides that is large and diverse. Publishing Authors By Initials

KA Thomsson,A Prakobphol,H Leffler,MS Reddy,MJ Levine,SJ Fisher,GC Hansson,

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The salivary mucin MG1 (MUC5B) carries a repertoire of unique oligosaccharides that is large and diverse. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Glycobiology

VOLUME: 12

Page Numbers: 1-14

Journal Abbreviation: Glycobiology

ISSN: 0959-6658

DAY: 29

MONTH: Jan

YEAR: 2002

The salivary mucin MG1 (MUC5B) carries a repertoire of unique oligosaccharides that is large and diverse. Information

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LANGUAGE: eng

NlmUniqueID: 9104124

The salivary mucin MG1 (MUC5B) carries a repertoire of unique oligosaccharides that is large and diverse. Keywords Mesh Terms:

KEYWORDS: Spectrometry, Mass, Matrix-Assisted Lase

MESH TERMS: chemistry

Chemical & Substance for Abstract: The salivary mucin MG1 (MUC5B) carries a repertoire of unique oligosaccharides that is large and diverse. Information

Substance Name: Oligosaccharides

Registry Number: 0

Grant and Affiliation Information for The salivary mucin MG1 (MUC5B) carries a repertoire of unique oligosaccharides that is large and diverse.

AFFILIATION: Department of Medical Biochemistry, Göteborg University, Medicinaregatan 9A, 413 90 Gothenburg, Sweden.

Country: England

AGENCY: United States NIDCR

GRANT: DE07244

ACRONYM: DE

MEDLINETA: Glycobiology

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