The rough energy landscape of superfolder GFP is linked to the chromophore.

The rough energy landscape of superfolder GFP is linked to the chromophore. Research Abstract Details 

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The rough energy landscape of superfolder GFP is linked to the chromophore. Abstract Text:

Benjamin T Andrews,Andrea R Schoenfish,Melinda Roy,Geoffrey Waldo,Patricia A Jennings,Benjamin T Andrews,Andrea R Schoenfish,Melinda Roy,Geoffrey Waldo,Patricia A Jennings,

Many green fluorescent protein (GFP) variants have been developed for use as fluorescent tags, and recently a superfolder GFP (sfGFP) has been developed as a robust folding reporter. This new variant shows increased stability and improved folding kinetics, as well as 100% recovery of native protein after denaturation. Here, we characterize sfGFP, and find that this variant exhibits hysteresis as unfolding and refolding equilibrium titration curves are non-coincident even after equilibration for more than eight half-lives as estimated from kinetic unfolding and refolding studies. This hysteresis is attributed to trapping in a native-like intermediate state. Mutational studies directed towards inhibiting chromophore formation indicate that the novel backbone cyclization is responsible for the hysteresis observed in equilibrium titrations of sfGFP. Slow equilibration and the presence of intermediates imply a rough landscape. However, de novo folding in the absence of the chromophore is dominated by a smoother energy landscape than that sampled during unfolding and refolding of the post-translationally modified polypeptide.

The rough energy landscape of superfolder GFP is linked to the chromophore. Publishing Authors By Initials

BT Andrews,AR Schoenfish,M Roy,G Waldo,PA Jennings,BT Andrews,AR Schoenfish,M Roy,G Waldo,PA Jennings,

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The rough energy landscape of superfolder GFP is linked to the chromophore. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of molecular biology

VOLUME: 373

Page Numbers: 476-90

Journal Abbreviation: J. Mol. Biol.

ISSN: 0022-2836

DAY: 15

MONTH: 08

YEAR: 2007

The rough energy landscape of superfolder GFP is linked to the chromophore. Information

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LANGUAGE: eng

NlmUniqueID: 2985088

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Grant and Affiliation Information for The rough energy landscape of superfolder GFP is linked to the chromophore.

AFFILIATION: Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA, 92093-0375, USA.

Country: England

AGENCY: United States NIGMS

GRANT: GM 54038

ACRONYM: GM

MEDLINETA: J Mol Biol

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