[The role of isomerized protein repair enzyme, PIMT, in cellular functions]

[The role of isomerized protein repair enzyme, PIMT, in cellular functions] Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

[The role of isomerized protein repair enzyme, PIMT, in cellular functions] Abstract Text:

Takemitsu Furuchi,Hiroshi Homma,Takemitsu Furuchi,Hiroshi Homma,Takemitsu Furuchi,Hiroshi Homma,

Proteins are subject to various types of spontaneous modifications that can disrupt their structures with sometimes adverse affects on biological activity. The formation of L-isoaspartyl (or D-aspartyl) residues, through either the deamidation of asparagine or dehydration of aspartate, is one of the most frequent types of deterioration occurring under physiological conditions. Protein L-isoaspartate/D-aspartate o-methyltransferase (PIMT) is a conserved and ubiquitous enzyme that participates in the repair of various isomerized proteins. PIMT catalyzes the transfer of the methyl group of S-adenosyl-L-methionine onto the alpha-carboxyl group of an L-isoaspartyl (or the beta-carboxyl group of an D-aspartyl) residue, which initiates the conversion of this residue to an L-aspartyl residue. PIMT-deficient mice have been shown to die at a mean age of 42 days from progressive epileptic seizures with grand mal and myoclonus. Although PIMT-deficiency clearly leads to the accumulation of isomerized proteins, it is currently unclear how this causes progressive epilepsy in PIMT-deficient mice. As a first step towards understanding this, we developed a new assay to measure PIMT activity in cell lysates. Additionally, we isolated PIMT knockdown cells from HEK293 cells that were stably transfected with a PIMT small interfering RNA expression vector. PIMT activities were significantly decreased in the PIMT knockdown cells, and analysis of the transfectants revealed that MEK and ERK were hyperactivated after cell stimulation with epidermal growth factor (EGF). These results indicate that the ability to repair L-isoaspartyl-(or D-aspartyl-) containing proteins is important for the maintenance of normal MEK-ERK signaling.

[The role of isomerized protein repair enzyme, PIMT, in cellular functions] Publishing Authors By Initials

T Furuchi,H Homma,T Furuchi,H Homma,T Furuchi,H Homma,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

[The role of isomerized protein repair enzyme, PIMT, in cellular functions] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Yakugaku zasshi : Journal of the Pharmaceutical So

VOLUME: 127

Page Numbers: 1927-36

Journal Abbreviation: Yakugaku Zasshi

ISSN: 0031-6903

DAY: 6

MONTH: Dec

YEAR: 2007

[The role of isomerized protein repair enzyme, PIMT, in cellular functions] Information

Number of References:

LANGUAGE: jpn

NlmUniqueID: 413613

[The role of isomerized protein repair enzyme, PIMT, in cellular functions] Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: [The role of isomerized protein repair enzyme, PIMT, in cellular functions] Information

Substance Name:

Registry Number:

Grant and Affiliation Information for [The role of isomerized protein repair enzyme, PIMT, in cellular functions]

AFFILIATION: Sch. of Pharmaceut. Sci., Kitasato Univ, 5-9-1 Shirogane, Minato-ku, Tokyo 108-8641, Japan.

Country: Japan

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Yakugaku Zasshi

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

The role of isomerized protein repair enzyme, PIMT, in cellular functions Related Publications

• A simple and effective treatment for stuttering: Speech practice without audience.
• A unique loop contributing to the structure of the ATP-binding cleft of skeletal muscle myosin communicates with the actin-binding site.
• Biochemistry of D-aspartate in mammalian cells.
• Characterization of a flagellar sheath component, PF60, and its structural gene in marine Vibrio.
• Characterization of circulating DNA in healthy human plasma.
• Cloning and characterization of motX, a Vibrio alginolyticus sodium-driven flagellar motor gene.
• Conformational changes in the unique loops bordering the ATP binding cleft of skeletal muscle myosin mediate energy transduction.
• Dynamic cardiac information from optical flow using four dimensional ultrasound.
• Effects of ultrasonic exposure parameters on myocardial lesions induced by high-intensity focused ultrasound.
• Essential regions of the lipopolysaccharide of Pseudomonas aeruginosa responsible for pyrogenicity and activation of the proclotting enzyme of horseshoe crabs. Comparison with antitumor, interferon-inducing and adjuvant activities.
• Flagellin-containing membrane vesicles excreted from Vibrio alginolyticus mutants lacking a polar-flagellar filament.
• Impact of valvular thickness on stroke recurrence in medically treated patients with stroke.
• Incidence of hypotonic-hyporesponsive episodes associated to the combined DTP/Hib vaccine used in Brazilian National Immunizations Program.
• Inhibition of phosphoinositide hydrolysis and cell growth of Swiss 3T3 cells by myristoylated phospholipase C inhibitor peptides.
• Involvement of selenoprotein P in the regulation of redox balance and myofibroblast viability in idiopathic pulmonary fibrosis.
• Lower urinary tract symptomatology: Its definition and confusion.
• Molecular cloning of a cDNA encoding mouse D-aspartate oxidase and functional characterization of its recombinant proteins by site-directed mutagenesis.
• Morphological Approach for the Functional Improvement of an Artificial Myocardial Assist Device using Shape Memory Alloy Fibres.
• Multimeric structure of the PomA/PomB channel complex in the Na+-driven flagellar motor of Vibrio alginolyticus.
• On the isolation of antigenic substances from Pseudomonas aeruginosa.
• Pneumoperitoneum recurring concomitantly with asthmatic exacerbation.
• Quantitative in silico analysis of the selectivity of graphitic carbon synthesized by different methods.
• Re: Validation of the overactive bladder symptom score J. G. Blaivas, G. Panagopoulos, J. P. Weiss And C. Somaroo J Urol 2007; 178: 543-547.
• Regulation of polar flagellar number by the flhF and flhG genes in Vibrio alginolyticus.
• Treatment of hypertension and other cardiovascular risk factors in patients with metabolic syndrome.
• Two modes of respiratory rhythm generation in the newborn rat brainstem-spinal cord preparation.
• [Diagnosis of extrauterine pregnancy from curettage materials.]
• [Not Available.]
• [Not Available.]
• [The role of isomerized protein repair enzyme, PIMT, in cellular functions]