The role of Ca2+ and Ca2+-activated phospholipid-dependent protein kinase in degranulation of human neutrophils.

The role of Ca2+ and Ca2+-activated phospholipid-dependent protein kinase in degranulation of human neutrophils. Research Abstract Details 

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The role of Ca2+ and Ca2+-activated phospholipid-dependent protein kinase in degranulation of human neutrophils. Abstract Text:

D Kang,H Tsuda,K Takeshige,Y Shibata,S Minakami,

The degranulation reactions of human neutrophils induced by 1-oleoyl-2-acetylglycerol (OAG), phorbol 12-myristate 13-acetate (PMA), and calcium ionophore A23187 or their combinations, were studied. OAG in the absence of the Ca2+-ionophore A23187 stimulated the releases of both lysozyme and lactoferrin, constituents of the specific granules, but did not stimulate the release of beta-glucuronidase, an enzyme of the azurophil granules. Electron microscopy revealed a selective decrease in the numbers of the specific granules in this case. The combined effects of A23187 at a concentration higher than 0.1 microM and OAG were essentially additive. W-7, known to be an inhibitor of both Ca2+-activated phospholipid-dependent protein kinase (C-kinase) and calmodulin, inhibited the degranulation induced by OAG or PMA, while it inhibited the reaction induced by A23187 less markedly. The release of lysozyme reached a plateau at about 0.1 microM A23187 and increased again at higher concentrations of A23187. The observations suggest that degranulation can be induced by the activation of the C-kinase, and the degranulation by A23187 at low concentrations may be due to the activation of the C-kinase; the effects of A23187 at high concentrations, however, could not be explained only in terms of the activation of the C-kinase.

The role of Ca2+ and Ca2+-activated phospholipid-dependent protein kinase in degranulation of human neutrophils. Publishing Authors By Initials

D Kang,H Tsuda,K Takeshige,Y Shibata,S Minakami,

For similar cells: cellular structures: intracellular space: cytoplasm: cytoplasmic structures: organelles: cytoplasmic vesicles: vacuoles research abstracts see: cells: cellular structures: intracellular space: cytoplasm: cytoplasmic structures: organelles: cytoplasmic vesicles: vacuoles research

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The role of Ca2+ and Ca2+-activated phospholipid-dependent protein kinase in degranulation of human neutrophils. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 98

Page Numbers: 1699-706

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Dec

YEAR: 1985

The role of Ca2+ and Ca2+-activated phospholipid-dependent protein kinase in degranulation of human neutrophils. Information

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LANGUAGE: eng

NlmUniqueID: 376600

The role of Ca2+ and Ca2+-activated phospholipid-dependent protein kinase in degranulation of human neutrophils. Keywords Mesh Terms:

KEYWORDS: Vacuoles

MESH TERMS: drug effects

Chemical & Substance for Abstract: The role of Ca2+ and Ca2+-activated phospholipid-dependent protein kinase in degranulation of human neutrophils. Information

Substance Name: Glucuronidase

Registry Number: EC 3.2.1.31

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Country: JAPAN

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MEDLINETA: J Biochem

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