The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster.

The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster. Abstract Text:

Ronald P van Rij,Maria-Carla Saleh,Bassam Berry,Catherine Foo,Andrew Houk,Christophe Antoniewski,Raul Andino,

Most organisms have evolved defense mechanisms to protect themselves from viruses and other pathogens. Arthropods lack the protein-based adaptive immune response found in vertebrates. Here we show that the central catalytic component of the RNA-induced silencing complex (RISC), the nuclease Argonaute 2 (Ago-2), is essential for antiviral defense in adult Drosophila melanogaster. Ago-2-defective flies are hypersensitive to infection with a major fruit fly pathogen, Drosophila C virus (DCV), and with Cricket Paralysis virus (CrPV). Increased mortality in ago-2 mutant flies was associated with a dramatic increase in viral RNA accumulation and virus titers. The physiological significance of this antiviral mechanism is underscored by our finding that DCV encodes a potent suppressor of RNA interference (RNAi). This suppressor binds long double-stranded RNA (dsRNA) and inhibits Dicer-2-mediated processing of dsRNA into short interfering RNA (siRNA), but does not bind short siRNAs or disrupt the microRNA (miRNA) pathway. Based on these results we propose that RNAi is a major antiviral immune defense mechanism in Drosophila.

The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster. Publishing Authors By Initials

RP van Rij,MC Saleh,B Berry,C Foo,A Houk,C Antoniewski,R Andino,

For similar proteins: viral proteins research abstracts see: proteins: viral proteins research

PUBMED ID PMID:

MEDLINE DATE:

The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Genes & development

VOLUME: 20

Page Numbers: 2985-95

Journal Abbreviation: Genes Dev.

ISSN: 0890-9369

DAY: 1

MONTH: Nov

YEAR: 2006

The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8711660

The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster. Keywords Mesh Terms:

KEYWORDS: Viral Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster. Information

Substance Name: RNA Helicases

Registry Number: EC 2.7.7.-

Grant and Affiliation Information for The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster.

AFFILIATION: Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, CA 94143, USA.

Country: United States

AGENCY: United States NIAID

GRANT: AI64738

ACRONYM: AI

MEDLINETA: Genes Dev

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster Related Publications

• A morpholino oligomer targeting highly conserved internal ribosome entry site sequence is able to inhibit multiple species of picornavirus.
• Engineering attenuated virus vaccines by controlling replication fidelity.
• Harnessing Endogenous miRNAs to Control Virus Tissue Tropism as a Strategy for Developing Attenuated Virus Vaccines.
• Poliovirus infection blocks ERGIC-to-Golgi trafficking and induces microtubule-dependent disruption of the Golgi complex.
• The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster.
• The silent treatment: RNAi as a defense against virus infection in mammals.