The responses of HT22 cells to the blockade of mitochondrial complexes and potential protective effect of selenium supplementation.

The responses of HT22 cells to the blockade of mitochondrial complexes and potential protective effect of selenium supplementation. Research Abstract Details 

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The responses of HT22 cells to the blockade of mitochondrial complexes and potential protective effect of selenium supplementation. Abstract Text:

Jun Panee,Wanyu Liu,Kyoko Nakamura,Marla J Berry,

Mitochondria are the major reactive oxygen species (ROS)--generating sites in mammalian cells. Blockade of complexes in the electron transport chain (ETC) increases the leakage of single electrons to O(2) and therefore increases ROS levels. Complexes I and III have been reported to be the major ROS-generating sites in mitochondria. In this study, using mouse hippocampal HT22 cells as in vitro model, we monitored the change of intracellular ROS level in response to the blockade of ETC at different complex, and measured changes of gene expression of antioxidant enzymes and phase II enzymes, also evaluated potential protective effect of selenium (Se) supplementation to the cells under this oxidative stress. In summary, our results showed that complex I was the major ROS-generating site in HT22 cells. Complex I blockade upregulated the mRNA levels of glutamylcysteine synthetase heavy and light chains, glutathione-S-transferases omega1 and alpha 2, hemoxygenase 1, thioredoxin reductase 1, and selenoprotein H. Unexpectedly, the expression of the enzymes that directly scavenge ROS decreased, including superoxide dismutases 1 and 2, glutathione peroxidase 1, and catalase. Se supplementation increased glutathione levels and glutathione peroxidase activity, indicating a potential protective role in oxidative stress caused by ETC blockade.

The responses of HT22 cells to the blockade of mitochondrial complexes and potential protective effect of selenium supplementation. Publishing Authors By Initials

J Panee,W Liu,K Nakamura,MJ Berry,

For similar inorganic chemicals: electrolytes: ions: anions: oxides: peroxides: superoxides research abstracts see: inorganic chemicals: electrolytes: ions: anions: oxides: peroxides: superoxides research

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The responses of HT22 cells to the blockade of mitochondrial complexes and potential protective effect of selenium supplementation. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: International journal of biological sciences

VOLUME: 3

Page Numbers: 335-41

Journal Abbreviation: Int. J. Biol. Sci.

ISSN: 1449-2288

DAY: 13

MONTH: 07

YEAR: 2007

The responses of HT22 cells to the blockade of mitochondrial complexes and potential protective effect of selenium supplementation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101235568

The responses of HT22 cells to the blockade of mitochondrial complexes and potential protective effect of selenium supplementation. Keywords Mesh Terms:

KEYWORDS: Superoxides

MESH TERMS: metabolism

Chemical & Substance for Abstract: The responses of HT22 cells to the blockade of mitochondrial complexes and potential protective effect of selenium supplementation. Information

Substance Name: Electron Transport Complex I

Registry Number: EC 1.6.5.3

Grant and Affiliation Information for The responses of HT22 cells to the blockade of mitochondrial complexes and potential protective effect of selenium supplementation.

AFFILIATION: Department of Cell & Molecular Biology, John A Burns Medical School, University of Hawaii, Honolulu, HI 96813, USA.

Country: Australia

AGENCY: United States NINDS

GRANT: R01-NS40302

ACRONYM: NS

MEDLINETA: Int J Biol Sci

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