[The regulatory effect of endogenous hydrogen sulfide on hypoxic pulmonary hypertension]

[The regulatory effect of endogenous hydrogen sulfide on hypoxic pulmonary hypertension] Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

[The regulatory effect of endogenous hydrogen sulfide on hypoxic pulmonary hypertension] Abstract Text:

Chunyu Zhang,Junbao Du,Dingfang Bu,Hui Yan,Xiuying Tang,Qin Si,Chaoshu Tang,

OBJECTIVE: To study the changes of endogenous H2S and the effect of exogenously applied H2S on hypoxic pulmonary hypertension (HPH). METHODS: Male Wistar rats were randomly divided into control group (n = 6), hypoxia group (n = 7) and hypoxia + NaHS group (n = 6). The rats that received hypoxia were put into a normobaric hypoxic chamber to respire hypoxic gas containing 10% (volume fraction) O2 for 6 hours everyday. NaHS solution was injected to rats of hypoxia + NaHS group at a dosage of 0.78 mg.kg-1 before hypoxic challenge. After 21 days of hypoxia, the mean pulmonary artery pressure (mPAP) was measured. The weight ratio of right ventricle vs left ventricle and septum [RV/(LV + SP)] was measured. The microstructure and ultrastructure changes in pulmonary small arteries were examined using elastin staining and transparent electron microscope respectively. And the activity of H2S generating enzymes in homogenates of the lung tissue and pulmonary artery was measured. The cystathionine gamma--lyase (CSE) mRNA in lung tissue was also measured using quantitative RT-PCR. RESULTS: Compared with rats in the control group, the mPAP increased 45.6% (P < 0.01) and the RV/(LV + SP) ratio increased 41% (P < 0.01) in rats of hypoxia group respectively. The relative medium thickness (RMT) and relative medium areas (RMA) increased 41% and 49% respectively (P all < 0.01) under optical microscope. The ultrastructure of the pulmonary small arteries also changed significantly. The H2S generating enzyme activity in lung tissue and in pulmonary artery decreased 52% and 54% respectively (P < 0.01). There were obviously negative correlations between the mPAP and H2S generating enzymes ativity. Relative CSE mRNA amount in lung tissue also decreased 53% (P < 0.01) in rats of hypoxia group compared with that of control group. The mPAP, and the RV/(LV + SP) ratio of rats in hypoxia + NaHS group decreased 31.2%, and 24% respectively compard with that of hypoxia group (P < 0.01). The RMT and RMA in rats of hypoxia + NaHS group also decreased 36% and 31% respectively (P < 0.01). In hypoxia + NaHS group, the H2S generating enzymes activity and relative CSE mRNA amount in the lung tissue increased 67.7% and 110% respectively compared with those in hypoxia group (P all < 0.01). CONCLUSION: Endogenous H2S was involved in the pathogenesis of rat's HPH. Exogenously applied H2s could exert protective effect during HPH.

[The regulatory effect of endogenous hydrogen sulfide on hypoxic pulmonary hypertension] Publishing Authors By Initials

C Zhang,J Du,D Bu,H Yan,X Tang,Q Si,C Tang,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

[The regulatory effect of endogenous hydrogen sulfide on hypoxic pulmonary hypertension] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Beijing da xue xue bao. Yi xue ban = Journal of Pe

VOLUME: 35

Page Numbers: 488-93

Journal Abbreviation: Beijing Da Xue Xue Bao

ISSN: 1671-167X

DAY: 6

MONTH: Oct

YEAR: 2003

[The regulatory effect of endogenous hydrogen sulfide on hypoxic pulmonary hypertension] Information

Number of References:

LANGUAGE: chi

NlmUniqueID: 101125284

[The regulatory effect of endogenous hydrogen sulfide on hypoxic pulmonary hypertension] Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: [The regulatory effect of endogenous hydrogen sulfide on hypoxic pulmonary hypertension] Information

Substance Name:

Registry Number:

Grant and Affiliation Information for [The regulatory effect of endogenous hydrogen sulfide on hypoxic pulmonary hypertension]

AFFILIATION: Department of Pediatrics, Peking University First Hospital, Beijing 100034, China.

Country: China

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Beijing Da Xue Xue Bao

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

The regulatory effect of endogenous hydrogen sulfide on hypoxic pulmonary hypertension Related Publications

• Dysfunction of myocardial and vascular taurine transport in spontaneously hypertensive rats.
• Levels of adrenomedullin and proadrenomedullin N-terminal 20 peptide in myocardium and aorta of spontaneously hypertensive rats and Wistar-Kyoto rats.
• Sidearm approach: a promising strategy for construction of bisoxazoline-based ligand library.
• Surface X-ray scattering system at the SRRC.
• [A discussion on Zhang Cigong's materia medica]
• [A pedigree with myotonic dystrophy: non-CTG, non-CCTG repeat expansion]
• [A rapid method for diagnosing alpha-thalassemia-1 of Southeast Asia type]
• [Achievements in the research on the prevention and treatment of malaria in China]
• [An experimental study on the effects of wild type p16 gene on the proliferation and metabolism of human keloid fibroblasts]
• [Axis registration and image interpolation of rotary scanning echocardiogram]
• [Changes in adrenomedullin and receptor activity-modifying protein 2 mRNA in myocardium and vessels during L-NNA-induced hypertension in rats]
• [Comparative study on three different methods in typing hepatitis C virus]
• [Effect of fluoroacetamide on cardiomyocytes of rat and the antidotal effect of acetamide]
• [Effect of urotensin II on the nitric oxide production in neonatal rat cardiomyocytes]
• [Effects of peptides originated from the proadrenomedullin on the production of nitric oxide in rat aorta]
• [Experiences of prevention and treatment of infectious diseases in Chinese Soviet Republic]
• [Expression of bone morphogenetic protein (BMP)-2, 4 in mouse embryonic tooth during the bud stage]
• [Histological and ultrastructural variations after radiofrequency for soft palate in porcines]
• [Penile reconstruction by using a scapular free flap]
• [Preliminary experimental study on treatment of portal hypertension with auxiliary partial orthotopic liver transplantation.]
• [Preventive effect of endothelin-1 pretreatment on hypoxia-induced injury in cultured neonatal rat cardiomyocytes]
• [Prospect of the foveola formation in the bovine trabecular bone under fatigue process]
• [Protective effect and mechanism of morphine on acute myocardial ischemia/reperfusion injury in rats]
• [Relationship between the genotype of Candida albicans and oral lichen planus]
• [Repair of eyelid defect with a prefabricated flap]
• [Role of neurokinin-1 receptor in lung injury in rats with acute necrotizing pancreatitis]
• [The effect of liposome-carried metallothionein on secondary venous ischemia-reperfusion injury in a rat flap]
• [The influence of the LPS from bacteroides fragilis on the secretion of IL-2 and IL-4 from the peripheral blood mononuclear cells of normal volunteers]
• [The initiation of G2/M checkpoint by diallyl disulfide requires the activation of p38 MAP kinase in HL-60 cells]
• [The regulatory effect of endogenous hydrogen sulfide on hypoxic pulmonary hypertension]