The regulation of integrin-mediated osteoblast focal adhesion and focal adhesion kinase expression by nanoscale topography.

The regulation of integrin-mediated osteoblast focal adhesion and focal adhesion kinase expression by nanoscale topography. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

The regulation of integrin-mediated osteoblast focal adhesion and focal adhesion kinase expression by nanoscale topography. Abstract Text:

Jung Yul Lim,Andrea D Dreiss,Zhiyi Zhou,Joshua C Hansen,Christopher A Siedlecki,Robert W Hengstebeck,Juan Cheng,Nicholas Winograd,Henry J Donahue,

An important consideration in developing physical biomimetic cell-stimulating cues is that the in vivo extracellular milieu includes nanoscale topographic interfaces. We investigated nanoscale topography regulation of cell functions using human fetal osteoblastic (hFOB) cell culture on poly(l-lactic acid) and polystyrene (50/50 w/w) demixed nanoscale pit textures (14, 29, and 45nm deep pits). Secondary ion mass spectroscopy revealed that these nanotopographic surfaces had similar surface chemistries to that of pure PLLA because of PLLA component surface segregation during spin casting. We observed that 14 and 29nm deep pit surfaces increased hFOB cell attachment, spreading, selective integrin subunit expression (e.g., alphav relative to alpha5, beta1, or beta3), focal adhesive paxillin protein synthesis and paxillin colocalization with cytoskeletal actin stress fibers, and focal adhesion kinase (FAK) and phosphorylated FAK (pY397) expression to a greater degree than did 45nm deep pits or flat PLLA surfaces. Considering the important role of integrin-mediated focal adhesion and intracellular signaling in anchorage-dependent cell function, our results suggest a mechanism by which nanostructured physical signals regulate cell function. Modulation of integrin-mediated focal adhesion and related cell signaling by altering nanoscale substrate topography will have powerful applications in biomaterials science and tissue engineering.

The regulation of integrin-mediated osteoblast focal adhesion and focal adhesion kinase expression by nanoscale topography. Publishing Authors By Initials

JY Lim,AD Dreiss,Z Zhou,JC Hansen,CA Siedlecki,RW Hengstebeck,J Cheng,N Winograd,HJ Donahue,

For similar investigative techniques: clinical laboratory techniques: culture techniques: tissue engineering research abstracts see: investigative techniques: clinical laboratory techniques: culture techniques: tissue engineering research

PUBMED ID PMID:

MEDLINE DATE:

The regulation of integrin-mediated osteoblast focal adhesion and focal adhesion kinase expression by nanoscale topography. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biomaterials

VOLUME: 28

Page Numbers: 1787-97

Journal Abbreviation: Biomaterials

ISSN: 0142-9612

DAY: 21

MONTH: 12

YEAR: 2006

The regulation of integrin-mediated osteoblast focal adhesion and focal adhesion kinase expression by nanoscale topography. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8100316

The regulation of integrin-mediated osteoblast focal adhesion and focal adhesion kinase expression by nanoscale topography. Keywords Mesh Terms:

KEYWORDS: Tissue Engineering

MESH TERMS: methods

Chemical & Substance for Abstract: The regulation of integrin-mediated osteoblast focal adhesion and focal adhesion kinase expression by nanoscale topography. Information

Substance Name: Focal Adhesion Protein-Tyrosine Kinases

Registry Number: EC 2.7.1.112

Grant and Affiliation Information for The regulation of integrin-mediated osteoblast focal adhesion and focal adhesion kinase expression by nanoscale topography.

AFFILIATION: Department of Orthopaedics and Rehabilitation, Division of Musculoskeletal Sciences, Center for Biomedical Devices and Functional Tissue Engineering, College of Medicine, Pennsylvania State University, Hershey, PA, USA.

Country: England

AGENCY: United States NIBIB

GRANT: EB002016-13

ACRONYM: EB

MEDLINETA: Biomaterials

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

The regulation of integrin-mediated osteoblast focal adhesion and focal adhesion kinase expression by nanoscale topography Related Publications

• ATP release mediates fluid flow-induced proliferation of human bone marrow stromal cells.
• Absence of all cyclovertical extraocular muscles in a child who has Apert syndrome.
• Acute identification of cranial burst fracture: comparison between CT and MR imaging findings.
• Alterations in Cx43 and OB-cadherin affect breast cancer cell metastatic potential.
• Bicarbonate and active site zinc modulate the self-peroxidation of bovine copper-zinc superoxide dismutase.
• Black bear femoral geometry and cortical porosity are not adversely affected by ageing despite annual periods of disuse (hibernation).
• Cell sensing and response to micro- and nanostructured surfaces produced by chemical and topographic patterning.
• Congenital hypothyroidism, dwarfism, and hearing impairment caused by a missense mutation in the mouse dual oxidase 2 gene, Duox2.
• Could you be more specific, please: Self-discrepancies, affect, and variation in specificity and relevance.
• Cryopreservation of embryos as a means of germ plasm conservation in sheep.
• Decreased bone turnover with balanced resorption and formation prevent cortical bone loss during disuse (hibernation) in grizzly bears (Ursus arctos horribilis).
• Effect of surface nanoscale topography on elastic modulus of individual osteoblastic cells as determined by atomic force microscopy.
• Failure properties and strain distribution analysis of meniscal attachments.
• Granulomatous myositis in association with chronic graft vs. host disease.
• Health care costs grow out of whack with GNP.
• Health care pricing seen as nonsensical.
• Health insurers seek cost parity with Medicare.
• Insulinlike growth factors: Potential therapeutic options for osteoporosis.
• Intraoperative heparin in addition to postoperative low-molecular-weight heparin for thromboprophylaxis in total knee replacement.
• Mechanically induced intracellular calcium waves in osteoblasts demonstrate calcium fingerprints in bone cell mechanotransduction.
• Mechanically stimulated osteocytes regulate osteoblastic activity via gap junctions.
• Osteitis of the spine.
• Osteoprotegrin and the bone homing and colonization potential of breast cancer cells.
• Surface energy effects on osteoblast spatial growth and mineralization.
• Susceptibility of the strawberry crown moth (Lepidoptera: Sesiidae) to entomopathogenic nematodes.
• The regulation of integrin-mediated osteoblast focal adhesion and focal adhesion kinase expression by nanoscale topography.
• Toxicity and potential dangers of cyclotrimethyl-enetrinitramine.
• Unusual mycotic infection of the lacrimal canaliculi and conjunctiva.
• Urge to gamble in a simulated gambling environment.
• White-light schlieren optics using bacteriorhodopsin as an adaptive image grid.