The Radiation-Induced Cell-Death Signaling Pathway is Activated by Concurrent Use of Cisplatin in Sequential Biopsy Specimens from Patients with Cervical Cancer.

The Radiation-Induced Cell-Death Signaling Pathway is Activated by Concurrent Use of Cisplatin in Sequential Biopsy Specimens from Patients with Cervical Cancer. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

The Radiation-Induced Cell-Death Signaling Pathway is Activated by Concurrent Use of Cisplatin in Sequential Biopsy Specimens from Patients with Cervical Cancer. Abstract Text:

Mayumi Iwakawa,Tatsuya Ohno,Kaori Imadome,Miyako Nakawatari,Ken-Ichi Ishikawa,Minako Sakai,Shingo Katoh,Hitoshi Ishikawa,Hirohiko Tsujii,Takashi Imai,Mayumi Iwakawa,Tatsuya Ohno,Kaori Imadome,Miyako Nakawatari,Ken-ichi Ishikawa,Minako Sakai,Shingo Katoh,Hitoshi Ishikawa,Hirohiko Tsujii,Takashi Imai,

Objective: To identify changes in gene expression related to the concurrent use of platinum compounds with radiotherapy, in the treatment of cervical cancer. Patients and Methods: Biopsy specimens were obtained from 39 patients with squamous cell carcinoma of the uterine cervix, before and during fractionated radiotherapy. Twenty patients were treated with radiotherapy (RT) alone, while 19 received the same radiotherapy plus concomitant chemotherapy with cisplatin (CRT). Changes in gene expression induced by treatment were investigated using single-color oligo-microarrays consisting of 44K human sequences. Paraffin-embedded samples were used to examine apoptosis and the expression of protein by treatment-responsive genes. Changes in mRNA expression were assessed for these genes by real-time reverse transcriptase-polymerase chain reaction. Aberrant genomic change (detected using microarray-based comparative genomic hybridization), human papillomavirus infection, and p53 status were also evaluated. Results: The expression of CDKN1A, BAX, TNFSF8, and RRM2B was consistently upregulated by CRT (9 Gy with a single administration of cisplatin). Similar expression changes were induced by RT (9 Gy) alone, although the variability between tumors was greater. Apoptotic cells were significantly increased in both groups. CRT significantly increased the numbers of cases with diffusely distributed CDKN1A-positive cells. Genetic losses at 2q33-ter and gains of 3q26-ter were detected in the samples with high frequency; 60% were positive for human papillomavirus DNA; and three tumors had deletions/mutations of the p53 gene. There was no difference in the incidence of these genomic changes between the groups, and no association was found with the changes in expression of CDKN1A, BAX, TNFSF8 or RRM2B. Conclusions: Using biopsy samples from pretreatment and midtreatment cervical tumors, we identified therapy-induced genes related to the cell death signaling pathway. CRT produced a homogenous pattern of changes in expression of known radiation-responsive genes.

The Radiation-Induced Cell-Death Signaling Pathway is Activated by Concurrent Use of Cisplatin in Sequential Biopsy Specimens from Patients with Cervical Cancer. Publishing Authors By Initials

M Iwakawa,T Ohno,K Imadome,M Nakawatari,K Ishikawa,M Sakai,S Katoh,H Ishikawa,H Tsujii,T Imai,M Iwakawa,T Ohno,K Imadome,M Nakawatari,K Ishikawa,M Sakai,S Katoh,H Ishikawa,H Tsujii,T Imai,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

The Radiation-Induced Cell-Death Signaling Pathway is Activated by Concurrent Use of Cisplatin in Sequential Biopsy Specimens from Patients with Cervical Cancer. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Cancer biology & therapy

VOLUME: 6

Page Numbers: 905-11

Journal Abbreviation: Cancer Biol. Ther.

ISSN: 1538-4047

DAY: 5

MONTH: 03

YEAR: 2007

The Radiation-Induced Cell-Death Signaling Pathway is Activated by Concurrent Use of Cisplatin in Sequential Biopsy Specimens from Patients with Cervical Cancer. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101137842

The Radiation-Induced Cell-Death Signaling Pathway is Activated by Concurrent Use of Cisplatin in Sequential Biopsy Specimens from Patients with Cervical Cancer. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: The Radiation-Induced Cell-Death Signaling Pathway is Activated by Concurrent Use of Cisplatin in Sequential Biopsy Specimens from Patients with Cervical Cancer. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for The Radiation-Induced Cell-Death Signaling Pathway is Activated by Concurrent Use of Cisplatin in Sequential Biopsy Specimens from Patients with Cervical Cancer.

AFFILIATION: RadGenomics Research Group, Research Center for Charged Particle Therapy, National Institute of Radiological Sciences, Chiba, Japan.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Cancer Biol Ther

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

The Radiation-Induced Cell-Death Signaling Pathway is Activated by Concurrent Use of Cisplatin in Sequential Biopsy Specimens from Patients with Cervical Cancer Related Publications

• A novel gastric-cancer-associated mucin antigen defined by a monoclonal antibody A3D4.
• Arabidopsis COLD SHOCK DOMAIN PROTEIN2 is a RNA chaperone that is regulated by cold and developmental signals.
• Chelation-mediated aqueous synthesis of metal oxyhydroxide and oxide nanostructures: combination of ligand-controlled oxidation and ligand-cooperative morphogenesis.
• Chemically modified siRNA prolonged RNA interference in renal disease.
• Effect of Eleutheroccocus senticosus Extract on Human Physical Working Capacity.
• Electroporation-mediated gene therapy.
• Estimation of bound quaternary ammoniumion to a rod-like polyion with a large alkyl residue.
• Existence of low-molecular-weight thiols in Caenorhabditis elegans demonstrated by HPLC-fluorescene detection utilizing 7-chloro-N-[2-(dimethylamino)ethyl]-2,1,3-benzoxadiazole-4-sulfonamide.
• Expression of MUC2 gene in gastric regenerative, metaplastic, and neoplastic epithelia.
• Expression of sulfated carbohydrate chain and core peptides of mucin detected by monoclonal antibodies in Barrett's esophagus and esophageal adenocarcinoma.
• Haplotype-based analysis of genes associated with risk of adverse skin reactions after radiotherapy in breast cancer patients.
• High prevalence of Helicobacter pylori infection in Japanese patients with membranous nephropathy.
• Hypoxanthine phosphoribosyltransferase activity in bovine embryos during the early embryonic development.
• Immunohistochemical detection of MUC2 mucin core protein in ulcerative colitis.
• Induction of preferential chemotaxis of unstimulated B-lymphocytes by 2-arachidonoylglycerol in immunized mice.
• Interferon gamma assay for detecting latent tuberculosis infection in rheumatoid arthritis patients during infliximab administration.
• Mesangial cell extracellular-superoxide dismutase production under the cell grow and extracellular matrix.
• Nampt/PBEF/Visfatin regulates insulin secretion in beta cells as a systemic NAD biosynthetic enzyme.
• Nodular fasciitis of the breast.
• Production and characterization of IgA monoclonal antibody against ovalbumin.
• Stroke risk in systolic and combined systolic and diastolic hypertension determined using ambulatory blood pressure. The Ohasama study.
• Synthesis of benzofurazan derivatization reagents for carboxylic acids in liquid chromatography/electrospray ionization-tandem mass spectrometry.
• The Radiation-Induced Cell-Death Signaling Pathway is Activated by Concurrent Use of Cisplatin in Sequential Biopsy Specimens from Patients with Cervical Cancer.
• The charge effects of the self-diffusion constant of bovine mercaptalbumin.
• The release of monovalent counterions by addition of divalent countemons to aqueous solutions of maleic acid copolymer.
• The release of monovalent counterions by addition of divalent counterions in coulombic interaction system.
• [Evaluation of new fourth-generation human immunodeficiency virus antigen and antibody detection assay with enzyme-linked fluorescent immunoassay]
• [Medical imaging: its medical economics and recent situation in Japan.]
• [Postoperative arrhythmia following cardiac surgery]
• [Small intestinal mucin antigen (SIMA)]