The protein network of bacterial motility.

The protein network of bacterial motility. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

The protein network of bacterial motility. Abstract Text:

Seesandra V Rajagopala, Titz,Johannes Goll,Jodi R Parrish,Katrin Wohlbold,Matthew T McKevitt,Timothy Palzkill,Hirotada Mori,Russell L Finley,Peter Uetz,

Motility is achieved in most bacterial species by the flagellar apparatus. It consists of dozens of different proteins with thousands of individual subunits. The published literature about bacterial chemotaxis and flagella documented 51 protein-protein interactions (PPIs) so far. We have screened whole genome two-hybrid arrays of Treponema pallidum and Campylobacter jejuni for PPIs involving known flagellar proteins and recovered 176 and 140 high-confidence interactions involving 110 and 133 proteins, respectively. To explore the biological relevance of these interactions, we tested an Escherichia coli gene deletion array for motility defects (using swarming assays) and found 159 gene deletion strains to have reduced or no motility. Comparing our interaction data with motility phenotypes from E. coli, Bacillus subtilis, and Helicobacter pylori, we found 23 hitherto uncharacterized proteins involved in motility. Integration of phylogenetic information with our interaction and phenotyping data reveals a conserved core of motility proteins, which appear to have recruited many additional species-specific components over time. Our interaction data also predict 18,110 interactions for 64 flagellated bacteria.

The protein network of bacterial motility. Publishing Authors By Initials

SV Rajagopala,B Titz,J Goll,JR Parrish,K Wohlbold,MT McKevitt,T Palzkill,H Mori,RL Finley,P Uetz,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: protein binding research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: protein binding research

PUBMED ID PMID:

MEDLINE DATE:

The protein network of bacterial motility. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecular systems biology

VOLUME: 3

Page Numbers: 128

Journal Abbreviation: Mol. Syst. Biol.

ISSN: 1744-4292

DAY: 31

MONTH: 07

YEAR: 2007

The protein network of bacterial motility. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101235389

The protein network of bacterial motility. Keywords Mesh Terms:

KEYWORDS: Protein Binding

MESH TERMS: physiology

Chemical & Substance for Abstract: The protein network of bacterial motility. Information

Substance Name: Bacterial Proteins

Registry Number: 0

Grant and Affiliation Information for The protein network of bacterial motility.

AFFILIATION: Institute of Genetics, Forschungszentrum Karlsruhe, Karlsruhe, Germany.

Country: England

AGENCY: United States NCRR

GRANT: RR18327

ACRONYM: RR

MEDLINETA: Mol Syst Biol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

The protein network of bacterial motility Related Publications

• High-throughput screening for protein-protein interactions using yeast two-hybrid arrays.
• Improving yeast two-hybrid screening systems.
• MPIDB: the microbial protein interaction database.
• The binary protein interactome of Treponema pallidum--the syphilis spirochete.
• The protein network of bacterial motility.