The protein kinase CK2 phosphorylates SNAP190 to negatively regulate SNAPC DNA binding and human U6 transcription by RNA polymerase III.

The protein kinase CK2 phosphorylates SNAP190 to negatively regulate SNAPC DNA binding and human U6 transcription by RNA polymerase III. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

The protein kinase CK2 phosphorylates SNAP190 to negatively regulate SNAPC DNA binding and human U6 transcription by RNA polymerase III. Abstract Text:

Liping Gu,Rhonda Husain-Ponnampalam,Susanne Hoffmann-Benning,R William Henry,

Human U6 small nuclear RNA gene transcription by RNA polymerase III requires the general transcription factor SNAP(C), which binds to human small nuclear RNA core promoter elements and nucleates pre-initiation complex assembly with the Brf2-TFIIIB complex. Multiple components in this pathway are phosphorylated by the protein kinase CK2, including the Bdp1 subunit of the Brf2-TFIIIB complex, and RNA polymerase III, with negative and positive outcomes for U6 transcription, respectively. However, a role for CK2 phosphorylation of SNAP(C) in U6 transcription has not been defined. In this report, we investigated the role of CK2 in modulating the transcriptional properties of SNAP(C) and demonstrate that within SNAP(C), CK2 phosphorylates the N-terminal half of the SNAP190 subunit at two regions (amino acids 20-63 and 514-545) that each contain multiple CK2 consensus sites. SNAP190 phosphorylation by CK2 inhibits both SNAP(C) DNA binding and U6 transcription activity. Mutational analyses of SNAP190 support a model wherein CK2 phosphorylation triggers an allosteric inhibition of the SNAP190 Myb DNA binding domain.

The protein kinase CK2 phosphorylates SNAP190 to negatively regulate SNAPC DNA binding and human U6 transcription by RNA polymerase III. Publishing Authors By Initials

L Gu,R Husain-Ponnampalam,S Hoffmann-Benning,RW Henry,

For similar genetic processes: gene expression: transcription, genetic research abstracts see: genetic processes: gene expression: transcription, genetic research

PUBMED ID PMID:

MEDLINE DATE:

The protein kinase CK2 phosphorylates SNAP190 to negatively regulate SNAPC DNA binding and human U6 transcription by RNA polymerase III. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 27887-96

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 1

MONTH: 08

YEAR: 2007

The protein kinase CK2 phosphorylates SNAP190 to negatively regulate SNAPC DNA binding and human U6 transcription by RNA polymerase III. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

The protein kinase CK2 phosphorylates SNAP190 to negatively regulate SNAPC DNA binding and human U6 transcription by RNA polymerase III. Keywords Mesh Terms:

KEYWORDS: Transcription, Genetic

MESH TERMS: chemistry

Chemical & Substance for Abstract: The protein kinase CK2 phosphorylates SNAP190 to negatively regulate SNAPC DNA binding and human U6 transcription by RNA polymerase III. Information

Substance Name: RNA Polymerase III

Registry Number: EC 2.7.7.-

Grant and Affiliation Information for The protein kinase CK2 phosphorylates SNAP190 to negatively regulate SNAPC DNA binding and human U6 transcription by RNA polymerase III.

AFFILIATION: Department of Biochemistry & Molecular Biology, Michigan State University, East Lansing, Michigan 48824, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: R01-GM59805

ACRONYM: GM

MEDLINETA: J Biol Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

The protein kinase CK2 phosphorylates SNAP190 to negatively regulate SNAPC DNA binding and human U6 transcription by RNA polymerase III Related Publications

• A prospective analysis of long-term quality of life after permanent I-125 brachytherapy for localised prostate cancer.
• Arabidopsis kinome: after the casting.
• Can a universal influenza immunization program reduce emergency department volume?
• Chemotherapeutic agents up-regulate the cytomegalovirus promoter: implications for bioluminescence imaging of tumor response to therapy.
• Comparison of diffuse reflectance fourier transform mid-infrared and near-infrared spectroscopy with grating-based near-infrared for the determination of fatty acids in forages.
• Detecting estrus in synchronized heifers-using tailpaint and an aerosol raddle.
• Diffusion tensor MR imaging tractography of the pyramidal tracts correlates with clinical motor function in children with congenital hemiparesis.
• Effect of amendment C:N ratio on plant richness, cover and metal content for acidic Pb and Zn mine tailings in Leadville, Colorado.
• Failed extubation in the neonatal intensive care unit.
• Fatty acid methyl esters in grasshopper eggs.
• Identifying risk factors for postpartum tobacco use.
• Integrated membrane filters for minimizing hydrodynamic flow and filtering in microfluidic devices.
• Intramyocardial transplantation of autologous CD34+ stem cells for intractable angina: a phase I/IIa double-blind, randomized controlled trial.
• Long term outcomes following iodine-125 monotherapy for localized prostate cancer: the cookridge 10 year results.
• Nasal catheter insertion for suction diathermy adenoidectomy.
• Noninvasive methods to identify early vascular access dysfunction.
• On the reliability of (13)C metabolic modeling with two-compartment neuronal-glial models.
• Regulation of 1D-myo-inositol-3-phosphate synthase in yeast.
• Retinoblastoma protein regulation by the COP9 signalosome.
• Role of glycogen synthase kinase-3 alpha in insulin action in cultured human skeletal muscle cells.
• Solamargine: The Main Glycoalkaloid from the Fruits of Solanum paludosum.
• Spectroscopic imaging with volume selection by unpaired adiabatic pi pulses: Theory and application.
• TRPM8 Axonal expression is decreased in painful human teeth with irreversible pulpitis and cold hyperalgesia.
• The emergence of yeast lipidomics.
• The grammatical morpheme deficit in moderate hearing impairment.
• The protein kinase CK2 phosphorylates SNAP190 to negatively regulate SNAPC DNA binding and human U6 transcription by RNA polymerase III.
• The unorthodox SNAP50 zinc finger domain contributes to cooperative promoter recognition by human SNAPC.
• Tissue-specific expression and regulation of GSK-3 in human skeletal muscle and adipose tissue.
• Undoing the misdescription of medicine.
• [Group therapy through graphic art.]