The prolyl isomerase Pin1 functions in mitotic chromosome condensation.

The prolyl isomerase Pin1 functions in mitotic chromosome condensation. Research Abstract Details 

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The prolyl isomerase Pin1 functions in mitotic chromosome condensation. Abstract Text:

Yu-Xin Xu,James L Manley,

The prolyl isomerase Pin1 plays important roles in numerous cellular processes. Here we provide evidence that Pin1 has an important function in chromosome condensation during mitosis. We first demonstrate that the interaction of Pin1 with chromatin is greatly elevated in G2/M phase and that this correlates with the presence on chromosomes of several mitotic phosphoproteins, especially topoisomerase (Topo) IIalpha. Inducible overexpression of Pin1 was shown to result in higher M phase-specific phosphorylation, while downregulation of Pin1 by siRNA treatment reduced phosphorylation of TopoIIalpha and other mitotic proteins. Furthermore, immunodepletion of Pin1 from mitotic cell extracts prevented such extracts from inducing chromosome condensation when added to S phase nuclei. Indeed, purified Pin1 and cdc2/cyclin B kinase were by themselves sufficient to induce condensation. This reflects the ability of Pin1 to increase TopoIIalpha phosphorylation by cdc2/cyclin B in vitro, which in turn dramatically increased formation of a TopoIIalpha/Pin1/DNA complex.

The prolyl isomerase Pin1 functions in mitotic chromosome condensation. Publishing Authors By Initials

YX Xu,JL Manley,

For similar biological phenomena, cell phenomena, and immunity: cell physiology: cell cycle: interphase: s phase research abstracts see: biological phenomena, cell phenomena, and immunity: cell physiology: cell cycle: interphase: s phase research

PUBMED ID PMID:

MEDLINE DATE:

The prolyl isomerase Pin1 functions in mitotic chromosome condensation. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Molecular cell

VOLUME: 26

Page Numbers: 287-300

Journal Abbreviation:

ISSN: 1097-2765

DAY: 27

MONTH: Apr

YEAR: 2007

The prolyl isomerase Pin1 functions in mitotic chromosome condensation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9802571

The prolyl isomerase Pin1 functions in mitotic chromosome condensation. Keywords Mesh Terms:

KEYWORDS: S Phase

MESH TERMS: genetics

Chemical & Substance for Abstract: The prolyl isomerase Pin1 functions in mitotic chromosome condensation. Information

Substance Name: DNA topoisomerase II alpha

Registry Number: EC 5.99.1.3

Grant and Affiliation Information for The prolyl isomerase Pin1 functions in mitotic chromosome condensation.

AFFILIATION: Department of Biological Sciences, Sherman Fairchild Building, Columbia University, New York, NY 10027, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM37971

ACRONYM: GM

MEDLINETA: Mol Cell

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