The post-polyketide synthase modification steps in the biosynthesis of the antitumor agent ansamitocin by Actinosynnema pretiosum.

The post-polyketide synthase modification steps in the biosynthesis of the antitumor agent ansamitocin by Actinosynnema pretiosum. Research Abstract Details 

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The post-polyketide synthase modification steps in the biosynthesis of the antitumor agent ansamitocin by Actinosynnema pretiosum. Abstract Text:

Peter Spiteller,Linquan Bai,Guangdong Shang,Brian J Carroll,Tin-Wein Yu,Heinz G Floss,

The functions of six genes in the ansamitocin biosynthetic gene cluster of Actinosynnema pretiosum have been investigated by gene inactivation and chemical analysis of the mutants. They encode a halogenase (asm12), a carbamoyltransferase (asm21), a 20-O-methyltransferase (asm7), a 3-O-acyltransferase (asm19), an epoxidase (asm11), and an N-methyltransferase (asm10), respectively, and are responsible for the six post-PKS modification steps in ansamitocin formation. Several of the enzymes have relaxed substrate specificities, resulting in multiple parallel pathways in a metabolic grid, albeit with a preferred sequence of reactions as listed above.

The post-polyketide synthase modification steps in the biosynthesis of the antitumor agent ansamitocin by Actinosynnema pretiosum. Publishing Authors By Initials

P Spiteller,L Bai,G Shang,BJ Carroll,TW Yu,HG Floss,

For similar macromolecular substances: multiprotein complexes: multienzyme complexes research abstracts see: macromolecular substances: multiprotein complexes: multienzyme complexes research

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The post-polyketide synthase modification steps in the biosynthesis of the antitumor agent ansamitocin by Actinosynnema pretiosum. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of the American Chemical Society

VOLUME: 125

Page Numbers: 14236-7

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 26

MONTH: Nov

YEAR: 2003

The post-polyketide synthase modification steps in the biosynthesis of the antitumor agent ansamitocin by Actinosynnema pretiosum. Information

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LANGUAGE: eng

NlmUniqueID: 7503056

The post-polyketide synthase modification steps in the biosynthesis of the antitumor agent ansamitocin by Actinosynnema pretiosum. Keywords Mesh Terms:

KEYWORDS: Multienzyme Complexes

MESH TERMS: metabolism

Chemical & Substance for Abstract: The post-polyketide synthase modification steps in the biosynthesis of the antitumor agent ansamitocin by Actinosynnema pretiosum. Information

Substance Name: ansamitocins

Registry Number: 69279-90-9

Grant and Affiliation Information for The post-polyketide synthase modification steps in the biosynthesis of the antitumor agent ansamitocin by Actinosynnema pretiosum.

AFFILIATION: Department of Chemistry, Box 351700, University of Washington, Seattle, WA 98195-1700, USA.

Country: United States

AGENCY: United States NCI

GRANT: CA 76461

ACRONYM: CA

MEDLINETA: J Am Chem Soc

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