The possibility of reducing xenoantigen levels with a novel gal 3'-sulfotransferase (GP3ST).

The possibility of reducing xenoantigen levels with a novel gal 3'-sulfotransferase (GP3ST). Research Abstract Details 

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The possibility of reducing xenoantigen levels with a novel gal 3'-sulfotransferase (GP3ST). Abstract Text:

Masaru Koma,Shuji Miyagawa,Koichi Honke,Rie Nakai,Shinichirou Miyoshi,Mitsunori Ohta,Hikaru Matsuda,Ryota Shirakura,Naoyuki Taniguchi,

Glycoprotein-3-sulfotransferase (GP3ST) is a key enzyme in downregulating the expression of Galalpha1,3Galbeta1,4GlcNAc-R (the alpha-Gal epitope), via enzymatic competition with an alpha1,3 galactosyltransferase (alpha1,3GT), such as alpha2,6 sialyltransferase (alpha2,6ST). In this study, we report the dominance of GP3ST over alpha1,3GT using transfected pig endothelial cell (PEC) lines. The introduction of the GP3ST gene into PEC suppresses its antigenicity with respect to normal human pooled serum (NHS), including the alpha-Gal epitope and the Hanganutziu-Deicher (H-D) antigen, and, in addition, reduces the susceptibility to NHS in complement-mediated cell lysis. Western and lectin blot analyses of the products of parental PEC and its transfectants indicated that proteins smaller than 66 kDa have a diminished reactivity with NHS and the IB4 lectin. The levels of the alpha-Gal epitope in neutral glycosphingolipids were also decreased in the GP3ST transfectants as detected in thin layer chromatography by immunostaining. These data indicate that GP3ST is very effective in reducing xenoepitope levels.

The possibility of reducing xenoantigen levels with a novel gal 3'-sulfotransferase (GP3ST). Publishing Authors By Initials

M Koma,S Miyagawa,K Honke,R Nakai,S Miyoshi,M Ohta,H Matsuda,R Shirakura,N Taniguchi,

For similar enzymes and coenzymes: enzymes: hydrolases: glycoside hydrolases: galactosidases: alpha-galactosidase research abstracts see: enzymes and coenzymes: enzymes: hydrolases: glycoside hydrolases: galactosidases: alpha-galactosidase research

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The possibility of reducing xenoantigen levels with a novel gal 3'-sulfotransferase (GP3ST). Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 131

Page Numbers: 517-22

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Apr

YEAR: 2002

The possibility of reducing xenoantigen levels with a novel gal 3'-sulfotransferase (GP3ST). Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

The possibility of reducing xenoantigen levels with a novel gal 3'-sulfotransferase (GP3ST). Keywords Mesh Terms:

KEYWORDS: alpha-Galactosidase

MESH TERMS: metabolism

Chemical & Substance for Abstract: The possibility of reducing xenoantigen levels with a novel gal 3'-sulfotransferase (GP3ST). Information

Substance Name: alpha-Galactosidase

Registry Number: EC 3.2.1.22

Grant and Affiliation Information for The possibility of reducing xenoantigen levels with a novel gal 3'-sulfotransferase (GP3ST).

AFFILIATION: Division of Organ Transplantation, Department of Regenerative Medicine, Osaka University Graduate School of Medicine, Yamadaoka, Suita, 565-0871, Japan.

Country: Japan

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MEDLINETA: J Biochem

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