The pH-dependent changes of intramolecular electron transfer on copper-containing nitrite reductase.

The pH-dependent changes of intramolecular electron transfer on copper-containing nitrite reductase. Research Abstract Details 

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The pH-dependent changes of intramolecular electron transfer on copper-containing nitrite reductase. Abstract Text:

K Kobayashi,S Tagawa, Deligeer,S Suzuki,

Electron transfer over 12.6 A from the type 1 copper (T1Cu) to the type 2 copper (T2Cu) was investigated in the copper-containing nitrite reductases from two denitrifying bacteria (Alcaligenes xylosoxidans GIFU 1051 and Achromobacter cycloclastes IAN 1013), following pulse radiolytical reduction of T1Cu. In the presence of nitrite, the rate constant for the intramolecular electron transfer of the enzyme from A. xylosoxidans decreased 1/2 fold to 9 x 10(2) s-1 (20 degrees C, pH 7.0) as compared to that for the same process in the absence of nitrite. However, the rate constant increased with decreasing pH to become the same (2 x 10(3) s-1) as that in the absence of nitrite at pH 6.0. A similar result was obtained for the enzyme from A. cycloclastes. The pH profiles of the two enzymes in the presence of nitrite are almost the same as that of the enzyme activity of nitrite reduction. This suggests that the intramolecular electron transfer process is closely linked to the following process of catalytic reduction of nitrite. The difference in redox potential (DeltaE) of T2Cu minus T1Cu was calculated from equilibrium data for the electron transfer. The pH-dependence of DeltaE was in accord with the equation: DeltaE = DeltaE(0)+0.058 log (Kr[H+]+[H+]2)/(K(0)+[H+]), where K(r) and K(0) are the proton dissociation constants for the oxidized and reduced states of T2Cu, respectively. These results raise the possibility that amino acid residues linked by the redox of T2Cu play important roles in the enzyme reaction, being located near T2Cu.

The pH-dependent changes of intramolecular electron transfer on copper-containing nitrite reductase. Publishing Authors By Initials

K Kobayashi,S Tagawa, Deligeer,S Suzuki,

For similar investigative techniques: chemistry, analytical: photometry: spectrophotometry research abstracts see: investigative techniques: chemistry, analytical: photometry: spectrophotometry research

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The pH-dependent changes of intramolecular electron transfer on copper-containing nitrite reductase. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 126

Page Numbers: 408-12

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Aug

YEAR: 1999

The pH-dependent changes of intramolecular electron transfer on copper-containing nitrite reductase. Information

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LANGUAGE: eng

NlmUniqueID: 376600

The pH-dependent changes of intramolecular electron transfer on copper-containing nitrite reductase. Keywords Mesh Terms:

KEYWORDS: Spectrophotometry

MESH TERMS: metabolism

Chemical & Substance for Abstract: The pH-dependent changes of intramolecular electron transfer on copper-containing nitrite reductase. Information

Substance Name: Nitrite Reductases

Registry Number: EC 1.7.-

Grant and Affiliation Information for The pH-dependent changes of intramolecular electron transfer on copper-containing nitrite reductase.

AFFILIATION: The Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka, 567-0047, Japan. kobayasi@sanken.osaka-u.ac.jp

Country: JAPAN

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MEDLINETA: J Biochem

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