The Par-3 NTD adopts a PB1-like structure required for Par-3 oligomerization and membrane localization.

The Par-3 NTD adopts a PB1-like structure required for Par-3 oligomerization and membrane localization. Research Abstract Details 

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The Par-3 NTD adopts a PB1-like structure required for Par-3 oligomerization and membrane localization. Abstract Text:

Wei Feng,Hao Wu,Ling-Nga Chan,Mingjie Zhang,

The evolutionarily conserved Par-3/Par-6/aPKC complex is essential for the establishment and maintenance of polarity of a wide range of cells. Both Par-3 and Par-6 are PDZ domain containing scaffold proteins capable of binding to polarity regulatory proteins. In addition to three PDZ domains, Par-3 also contains a conserved N-terminal oligomerization domain (NTD) that is essential for proper subapical membrane localization and consequently the functions of Par-3. The molecular basis of NTD-mediated Par-3 membrane localization is poorly understood. Here, we describe the structure of a monomeric form of the Par-3 NTD. Unexpectedly, the domain adopts a PB1-like fold with both type-I and type-II structural features. The Par-3 NTD oligomerizes into helical filaments via front-to-back interactions. We further demonstrate that the NTD-mediated membrane localization of Par-3 in MDCK cells is solely attributed to its oligomerization capacity. The data presented in this study suggest that the Par-3 NTD is likely to facilitate the assembly of higher-order Par-3/Par-6/aPKC complex with increased avidities in targeting the complex to the subapical membrane domain and in binding to other polarity-regulating proteins.

The Par-3 NTD adopts a PB1-like structure required for Par-3 oligomerization and membrane localization. Publishing Authors By Initials

W Feng,H Wu,LN Chan,M Zhang,

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The Par-3 NTD adopts a PB1-like structure required for Par-3 oligomerization and membrane localization. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The EMBO journal

VOLUME: 26

Page Numbers: 2786-96

Journal Abbreviation: EMBO J.

ISSN: 0261-4189

DAY: 3

MONTH: 05

YEAR: 2007

The Par-3 NTD adopts a PB1-like structure required for Par-3 oligomerization and membrane localization. Information

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LANGUAGE: eng

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Grant and Affiliation Information for The Par-3 NTD adopts a PB1-like structure required for Par-3 oligomerization and membrane localization.

AFFILIATION: Department of Biochemistry, Molecular Neuroscience Center, The Hong Kong University of Science and Technology, Kowloon, Hong Kong.

Country: England

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MEDLINETA: EMBO J

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