The Outer membrane protein OmpA of Mannheimia haemolytica A1 is involved in the binding of fibronectin.

The Outer membrane protein OmpA of Mannheimia haemolytica A1 is involved in the binding of fibronectin. Research Abstract Details 

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The Outer membrane protein OmpA of Mannheimia haemolytica A1 is involved in the binding of fibronectin. Abstract Text:

Reggie Y C Lo,Lea S Sorensen,

An enzyme-linked immunosorbent assay using bovine fibronectin as the substrate was used to demonstrate that Mannheimia haemolytica A1 binds to fibronectin. This binding to fibronectin was specific as no binding was observed with bovine fibrinogen. The binding to fibronectin was not observed if the M. haemolytica A1 cells were pretreated with trypsin or proteinase K, suggesting that it involved a protein molecule on the cell surface. Interestingly, the fibronectin-binding activity was found to be higher in an acapsular mutant compared with its parent strain. The fibronectin-binding protein was shown to be present in the outer membrane fraction of M. haemolytica A1. A 45 kDa outer membrane protein that binds to fibronectin was identified by Far-Western immunoblot analysis. This protein was purified and subjected to MS matrix-assisted laser desorption ionization time-of-flight analysis. The results identified it to be outer membrane OmpA based on comparison with the M. haemolytica A1 genomic sequence.

The Outer membrane protein OmpA of Mannheimia haemolytica A1 is involved in the binding of fibronectin. Publishing Authors By Initials

RY Lo,LS Sorensen,

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The Outer membrane protein OmpA of Mannheimia haemolytica A1 is involved in the binding of fibronectin. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: FEMS microbiology letters

VOLUME: 274

Page Numbers: 226-31

Journal Abbreviation: FEMS Microbiol. Lett.

ISSN: 0378-1097

DAY: 30

MONTH: 06

YEAR: 2007

The Outer membrane protein OmpA of Mannheimia haemolytica A1 is involved in the binding of fibronectin. Information

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LANGUAGE: eng

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Grant and Affiliation Information for The Outer membrane protein OmpA of Mannheimia haemolytica A1 is involved in the binding of fibronectin.

AFFILIATION: Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada.

Country: England

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MEDLINETA: FEMS Microbiol Lett

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