The Osterix Transcription Factor Down-Regulates Interleukin-1{alpha} Expression in Mouse Osteosarcoma Cells.

The Osterix Transcription Factor Down-Regulates Interleukin-1{alpha} Expression in Mouse Osteosarcoma Cells. Research Abstract Details 

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The Osterix Transcription Factor Down-Regulates Interleukin-1{alpha} Expression in Mouse Osteosarcoma Cells. Abstract Text:

Ying Cao,Shu-Fang Jia,Geetika Chakravarty,Benoit de Crombrugghe,Eugenie S Kleinerman,Ying Cao,Shu-Fang Jia,Geetika Chakravarty,Benoit de Crombrugghe,Eugenie S Kleinerman,

K7M2 mouse osteosarcoma cells form lytic tumors and are deficient in osterix (Osx), a zinc finger-containing transcription factor required for osteoblast differentiation and bone formation. Our previous studies showed that replacement of Osx suppresses lytic bone destruction. Cytokines, including interleukin (IL)-1alpha, IL-6, IL-11, and prostaglandin E2, have been shown to stimulate osteoclast activity. We showed that IL-1alpha production by K7M2 cells was significantly suppressed following Osx transfection through a transcription-mediated mechanism. Osx had no effect on IL-6, IL-11, or prostaglandin E2. Site-directed mutagenesis and chromatin immunoprecipitation indicated that Osx down-regulated IL-1alpha through an Sp1-binding site on the IL-1alpha promoter. Inhibiting Osx by small interfering RNA in two cell lines (Dunn and DLM8) that expressed high levels of Osx led to enhanced IL-1alpha promoter activity and protein production and altered the phenotype from blastic to lytic. These data suggest that Osx down-regulates IL-1alpha expression in mouse osteosarcoma cells via transcriptional repression of IL-1alpha and this may in turn affect the lytic activity of the tumor cells. (Mol Cancer Res 2008;6(1):119-26).

The Osterix Transcription Factor Down-Regulates Interleukin-1{alpha} Expression in Mouse Osteosarcoma Cells. Publishing Authors By Initials

Y Cao,SF Jia,G Chakravarty,B de Crombrugghe,ES Kleinerman,Y Cao,SF Jia,G Chakravarty,B de Crombrugghe,ES Kleinerman,

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The Osterix Transcription Factor Down-Regulates Interleukin-1{alpha} Expression in Mouse Osteosarcoma Cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Molecular cancer research : MCR

VOLUME: 6

Page Numbers: 119-26

Journal Abbreviation: Mol. Cancer Res.

ISSN: 1541-7786

DAY: 31

MONTH: Jan

YEAR: 2008

The Osterix Transcription Factor Down-Regulates Interleukin-1{alpha} Expression in Mouse Osteosarcoma Cells. Information

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LANGUAGE: eng

NlmUniqueID: 101150042

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AFFILIATION: Division of Pediatrics, The University of Texas M. D. Anderson Cancer Center, Unit 87, 1515 Holcombe Boulevard, Houston, TX 77030. ekleiner@mdanderson.org.

Country: United States

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MEDLINETA: Mol Cancer Res

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