The novel ER membrane protein PRO41 is essential for sexual development in the filamentous fungus Sordaria macrospora.

The novel ER membrane protein PRO41 is essential for sexual development in the filamentous fungus Sordaria macrospora. Research Abstract Details 

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The novel ER membrane protein PRO41 is essential for sexual development in the filamentous fungus Sordaria macrospora. Abstract Text:

Silke Busch,Gerhard H Braus,

The filamentous fungus Sordaria macrospora develops complex fruiting bodies (perithecia) to propagate its sexual spores. Here, we present an analysis of the sterile mutant pro41 that is unable to produce mature fruiting bodies. The mutant carries a deletion of 4 kb and is complemented by the pro41 open reading frame that is contained within the region deleted in the mutant. In silico analyses predict PRO41 to be an endoplasmic reticulum (ER) membrane protein, and a PRO41-EGFP fusion protein colocalizes with ER-targeted DsRED. Furthermore, Western blot analysis shows that the PRO41-EGFP fusion protein is present in the membrane fraction. A fusion of the predicted N-terminal signal sequence of PRO41 with EGFP is secreted out of the cell, indicating that the signal sequence is functional. pro41 transcript levels are upregulated during sexual development. This increase in transcript levels was not observed in the sterile mutant pro1 that lacks a transcription factor gene. Moreover, microarray analysis of gene expression in the mutants pro1, pro41 and the pro1/41 double mutant showed that pro41 is partly epistatic to pro1. Taken together, these data show that PRO41 is a novel ER membrane protein essential for fruiting body formation in filamentous fungi.

The novel ER membrane protein PRO41 is essential for sexual development in the filamentous fungus Sordaria macrospora. Publishing Authors By Initials

S Busch,GH Braus,

For similar fungi: ascomycota: sordariales research abstracts see: fungi: ascomycota: sordariales research

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The novel ER membrane protein PRO41 is essential for sexual development in the filamentous fungus Sordaria macrospora. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecular microbiology

VOLUME: 64

Page Numbers: 923-37

Journal Abbreviation: Mol. Microbiol.

ISSN: 0950-382X

DAY: 3

MONTH: May

YEAR: 2007

The novel ER membrane protein PRO41 is essential for sexual development in the filamentous fungus Sordaria macrospora. Information

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LANGUAGE: eng

NlmUniqueID: 8712028

The novel ER membrane protein PRO41 is essential for sexual development in the filamentous fungus Sordaria macrospora. Keywords Mesh Terms:

KEYWORDS: Sordariales

MESH TERMS: growth & development

Chemical & Substance for Abstract: The novel ER membrane protein PRO41 is essential for sexual development in the filamentous fungus Sordaria macrospora. Information

Substance Name: Green Fluorescent Proteins

Registry Number: 147336-22-9

Grant and Affiliation Information for The novel ER membrane protein PRO41 is essential for sexual development in the filamentous fungus Sordaria macrospora.

AFFILIATION: Lehrstuhl für Allgemeine und Molekulare Botanik, Ruhr-Universität Bochum, Bochum, Germany.

Country: England

AGENCY: United States NIGMS

GRANT: P01 GM068087

ACRONYM: GM

MEDLINETA: Mol Microbiol

REFSOURCE: Mol Microbiol. 2007 May;64(4):873-6

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