The novel endocannabinoid receptor GPR55 is activated by atypical cannabinoids but does not mediate their vasodilator effects.

The novel endocannabinoid receptor GPR55 is activated by atypical cannabinoids but does not mediate their vasodilator effects. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

The novel endocannabinoid receptor GPR55 is activated by atypical cannabinoids but does not mediate their vasodilator effects. Abstract Text:

D G Johns,D J Behm,D J Walker,Z Ao,E M Shapland,D A Daniels,M Riddick,S Dowell,P C Staton,P Green,U Shabon,W Bao,N Aiyar,T-L Yue,A J Brown,A D Morrison,S A Douglas,

BACKGROUND AND PURPOSE: Atypical cannabinoids are thought to cause vasodilatation through an as-yet unidentified 'CBx' receptor. Recent reports suggest GPR55 is an atypical cannabinoid receptor, making it a candidate for the vasodilator 'CBx' receptor. The purpose of the present study was to test the hypothesis that human recombinant GPR55 is activated by atypical cannabinoids and mediates vasodilator responses to these agents. EXPERIMENTAL APPROACH: Human recombinant GPR55 was expressed in HEK293T cells and specific GTPgammaS activity was monitored as an index of receptor activation. In GPR55-deficient and wild-type littermate control mice, in vivo blood pressure measurement and isolated resistance artery myography were used to determine GPR55 dependence of atypical cannabinoid-induced haemodynamic and vasodilator responses. KEY RESULTS: Atypical cannabinoids O-1602 and abnormal cannabidiol both stimulated GPR55-dependent GTPgammaS activity (EC50 approximately 2 nM), whereas the CB1 and CB2-selective agonist WIN 55,212-2 showed no effect in GPR55-expressing HEK293T cell membranes. Baseline mean arterial pressure and heart rate were not different between WT and GPR55 KO mice. The blood pressure-lowering response to abnormal cannabidiol was not different between WT and KO mice (WT 20+/-2%, KO 26+/-5% change from baseline), nor was the vasodilator response to abnormal cannabidiol in isolated mesenteric arteries (IC50 approximately 3 micro M for WT and KO). The abnormal cannabidiol vasodilator response was antagonized equivalently by O-1918 in both strains. CONCLUSIONS: These results demonstrate that while GPR55 is activated by atypical cannabinoids, it does not appear to mediate the vasodilator effects of these agents.

The novel endocannabinoid receptor GPR55 is activated by atypical cannabinoids but does not mediate their vasodilator effects. Publishing Authors By Initials

DG Johns,DJ Behm,DJ Walker,Z Ao,EM Shapland,DA Daniels,M Riddick,S Dowell,PC Staton,P Green,U Shabon,W Bao,N Aiyar,TL Yue,AJ Brown,AD Morrison,SA Douglas,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

The novel endocannabinoid receptor GPR55 is activated by atypical cannabinoids but does not mediate their vasodilator effects. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: British journal of pharmacology

VOLUME: 152

Page Numbers: 825-31

Journal Abbreviation: Br. J. Pharmacol.

ISSN: 0007-1188

DAY: 20

MONTH: 08

YEAR: 2007

The novel endocannabinoid receptor GPR55 is activated by atypical cannabinoids but does not mediate their vasodilator effects. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7502536

The novel endocannabinoid receptor GPR55 is activated by atypical cannabinoids but does not mediate their vasodilator effects. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: The novel endocannabinoid receptor GPR55 is activated by atypical cannabinoids but does not mediate their vasodilator effects. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for The novel endocannabinoid receptor GPR55 is activated by atypical cannabinoids but does not mediate their vasodilator effects.

AFFILIATION: GlaxoSmithKline, Cardiovascular and Urogenital Center for Excellence in Drug Discovery, Vascular Biology and Thrombosis, King of Prussia, PA 19406, USA. Douglas.G.Johns@gsk.com

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Br J Pharmacol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

The novel endocannabinoid receptor GPR55 is activated by atypical cannabinoids but does not mediate their vasodilator effects Related Publications

• Adenoviral vectors for gene therapy.
• Alcohol dependence is independently associated with sepsis, septic shock, and hospital mortality among adult intensive care unit patients.
• Blastocyst-derived trophoblast stem cells from the rhesus monkey.
• Cell death, remodeling, and repair in chronic obstructive pulmonary disease?
• Consumer acceptable risk: how cigarette companies have responded to accusations that their products are defective.
• Control of ovulation in cycling mares with ovarian steroids and prostaglandin.
• Controlled ligand display on a symmetrical protein-cage architecture through mixed assembly.
• Converging epistemologies: critical issues in Canadian Inuit childbirth and pregnancy.
• Cross-linkage of hydroxyapatite/gelatin nanocomposite using imide-based zero-length cross-linker.
• Culture-negative Bartonella endocarditis in a patient with renal failure: the value of molecular methods in diagnosis.
• Frequency of insomnia report in patients with obstructive sleep apnoea hypopnea syndrome (OSAHS).
• Functional status in patients with narcolepsy.
• Fuzzy clustering of CT images for the measurement of hydrocephalus associated with tuberculous meningitis.
• High-density targeting of a viral multifunctional nanoplatform to a pathogenic, biofilm-forming bacterium.
• Left ventricular hypertrophy in athletes: morphologic features and clinical correlates.
• Lipid A fraction of LPS induces a discrete MAPK activation in acute lung injury.
• MUC1 is involved in trophoblast transendothelial migration.
• Measurement of plasma-derived substance P: biological, methodological, and statistical considerations.
• Metabolic flux analysis for optimizing the specific growth rate of recombinant Aspergillus niger.
• Plasma oxytocin concentrations in cyclic mares and sexually aroused stallions.
• Predictors of hepatitis B vaccination in women prisoners in two prisons in England.
• Preliminary findings of an intervention integrating modified directly observed therapy and risk reduction counseling.
• Relationship between the ability of oral streptococci to interact with platelet glycoprotein Ibalpha and with the salivary low-molecular-weight mucin, MG2.
• Sequence variation in alpha-methylacyl-CoA racemase and risk of early-onset and familial prostate cancer.
• Synthetic control over magnetic moment and exchange bias in all-oxide materials encapsulated within a spherical protein cage.
• Target-assembled ExciProbes: Application to DNA Detection at the Level of PCR Product and Plasmid DNA.
• Targeting of cancer cells with ferrimagnetic ferritin cage nanoparticles.
• The novel endocannabinoid receptor GPR55 is activated by atypical cannabinoids but does not mediate their vasodilator effects.
• The outcome of twin pregnancies in mares.
• The use of radioreceptor assay for the detection of pregnancy in the mare.