The nematode neuropeptide, AF2 (KHEYLRF-NH2), increases voltage-activated calcium currents in Ascaris suum muscle.

The nematode neuropeptide, AF2 (KHEYLRF-NH2), increases voltage-activated calcium currents in Ascaris suum muscle. Research Abstract Details 

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The nematode neuropeptide, AF2 (KHEYLRF-NH2), increases voltage-activated calcium currents in Ascaris suum muscle. Abstract Text:

S Verma,A P Robertson,R J Martin,

BACKGROUND AND PURPOSE: Resistance to all the classes of anti-nematodal drugs like the benzimidazoles, cholinergic agonists and avermectins, has now been recorded in parasites of animals and/or humans. The development of novel anthelmintics is an urgent and imperative need. Receptors of nematode neuropeptides have been suggested to be suitable target sites for novel anthelmintic drugs. EXPERIMENTAL APPROACH: To investigate the effect of AF2 on calcium-currents in Ascaris suum somatic muscle cells we employed the two-micropipette current-clamp and voltage-clamp techniques and a brief application of AF2. KEY RESULTS: Here we report the isolation of voltage-activated, transient, inward calcium currents. These currents are similar in characteristics to Caenorhabditis elegans UNC-2 type currents, non-L-type calcium currents. Following a 2-minute application of 1 microM AF2 , there was a significant long-lasting increase in the transient inward calcium current; AF2 increased the maximum current (from -84 nA to -158 nA) by shifting the threshold in the hyperpolarising direction (V (50) changed from -7.2 to -12.8 mV) and increasing the maximum conductance change from 1.91 to 2.94 microS. CONCLUSION AND IMPLICATIONS: These studies demonstrate a mechanism by which AF2 increased the excitability of the neuromuscular system by modulating calcium currents in nematodes. A selective small molecule agonist of the AF2 receptor is predicted to increase the contraction and act synergistically with cholinergic anthelmintics and could counter resistance to these compounds.

The nematode neuropeptide, AF2 (KHEYLRF-NH2), increases voltage-activated calcium currents in Ascaris suum muscle. Publishing Authors By Initials

S Verma,AP Robertson,RJ Martin,

For similar peptides: neuropeptides research abstracts see: peptides: neuropeptides research

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The nematode neuropeptide, AF2 (KHEYLRF-NH2), increases voltage-activated calcium currents in Ascaris suum muscle. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: British journal of pharmacology

VOLUME: 151

Page Numbers: 888-99

Journal Abbreviation: Br. J. Pharmacol.

ISSN: 0007-1188

DAY: 21

MONTH: 05

YEAR: 2007

The nematode neuropeptide, AF2 (KHEYLRF-NH2), increases voltage-activated calcium currents in Ascaris suum muscle. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7502536

The nematode neuropeptide, AF2 (KHEYLRF-NH2), increases voltage-activated calcium currents in Ascaris suum muscle. Keywords Mesh Terms:

KEYWORDS: Neuropeptides

MESH TERMS: pharmacology

Chemical & Substance for Abstract: The nematode neuropeptide, AF2 (KHEYLRF-NH2), increases voltage-activated calcium currents in Ascaris suum muscle. Information

Substance Name: AF2 neuropeptide

Registry Number: 146269-94-5

Grant and Affiliation Information for The nematode neuropeptide, AF2 (KHEYLRF-NH2), increases voltage-activated calcium currents in Ascaris suum muscle.

AFFILIATION: Department of Biomedical Science, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.

Country: England

AGENCY: United States NIAID

GRANT: R01 AI047194

ACRONYM: AI

MEDLINETA: Br J Pharmacol

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