[The mutation of anti-CD3 antibody (HIT3a) gene and its expression]

[The mutation of anti-CD3 antibody (HIT3a) gene and its expression] Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

[The mutation of anti-CD3 antibody (HIT3a) gene and its expression] Abstract Text:

Y Xu,D Xiong,C Yang,Z Lai,H Liu,X He,H Peng,X Shao,C Xu,X Liao,Y Xie,Z Zhu,

OBJECTIVE: To improve the expression of anti-CD(3) single chain Fv (scFv) by site mutation and identify its biological activity. METHODS: Anti-CD(3) scFv gene was mutated by PCR, the target clones were screened by both the fingerprints of DNA restriction endonuclease digestion and Western blot, the antigen-binding activity of scFv was examined by FACS, competitive inhibition was performed with (125)I-labeled HIT3a and the cytotoxic effect mediated by the anti-CD(3) scFv-activated T lymphocytes was analyzed by (51)Cr-released assays. RESULTS: The DNA sequencing showed that the 6th amino acid of the anti-CD(3) antibody (HIT3a) heavy chain gene was mutated from E (GAG) to Q (CAG). The expression of mutated anti-CD(3) scFv (m2) was increased by 100 times higher than that of the parent scFv, and there was no difference in the Jurkat cell (CD(3)(+))-binding activity between the (m2) and parent scFv. The preliminary results of competitive assays showed that m2 could partially block the sites of CD(3)(+) Jurkat cells where the parent antibody bound to. Cytotoxicity assays demonstrated that CD(3)AK cells induced by IL-2 and m2 showed stronger cytotoxic effect than that of LAK cells induced by IL-2 alone in vitro. CONCLUSION: By site mutation, a high expression fragment m2 of anti-CD(3) scFv antibody was obtained. The results of some experiments indicated that m2 could bind to CD(3)(+) Jurkat cells, furthermore, by co-stimulated with IL-2, it could activate peripheral T lymphocytes and induce CD(3)AK cytotoxic effect.

[The mutation of anti-CD3 antibody (HIT3a) gene and its expression] Publishing Authors By Initials

Y Xu,D Xiong,C Yang,Z Lai,H Liu,X He,H Peng,X Shao,C Xu,X Liao,Y Xie,Z Zhu,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

[The mutation of anti-CD3 antibody (HIT3a) gene and its expression] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Zhonghua xue ye xue za zhi = Zhonghua xueyexue zaz

VOLUME: 22

Page Numbers: 252-5

Journal Abbreviation: Zhonghua Xue Ye Xue Za Zhi

ISSN: 0253-2727

DAY: 5

MONTH: May

YEAR: 2001

[The mutation of anti-CD3 antibody (HIT3a) gene and its expression] Information

Number of References:

LANGUAGE: chi

NlmUniqueID: 8212398

[The mutation of anti-CD3 antibody (HIT3a) gene and its expression] Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: [The mutation of anti-CD3 antibody (HIT3a) gene and its expression] Information

Substance Name:

Registry Number:

Grant and Affiliation Information for [The mutation of anti-CD3 antibody (HIT3a) gene and its expression]

AFFILIATION: State Key Laboratory of Experimental Hematology, Institute of Hematology, CAMS&PUMC, Tianjin 300020, China.

Country: China

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Zhonghua Xue Ye Xue Za Zhi

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

The mutation of anti-CD3 antibody HIT3a gene and its expression Related Publications

• Cell signaling by imidazoline-1 receptor candidate, IRAS, and the nischarin homologue.
• Cloning and expression of xeroderma pigmentosum complementation group A cDNA and the identification of its recombinant protein.
• Modification of glycosylation reduces microvilli on rat liver epithelial cells.
• [Aberrant neuronal expression of mitotic protein, tau and Bax in the rat brain after injection of Abeta(25-35) into the amygdala]
• [Application of expressed sequence tag (EST) in parasite genome research]
• [Clinical investigation of the correlation between blood concentration of lactic acid and tissue oxygenation in severely burned patients]
• [Combined detection of K-ras mutation and CA19-9 level in plasma of patients with pancreatic cancer]
• [Comparison of endoscopic irsection and vaporization for superficial bladder cancer]
• [Construction of the prokaryotic expression vector of MTB lhp gene and its expression]
• [Diagnosis and treatment of adrenal medullary hyperplasia (with report of 8 cases)]
• [Effects of Nomega-nitro-L-arginine on photoreceptor apoptosis in inherited retinal degeneration of RCS rats]
• [Effects of lamivudine on cirrhotic patients following chronic hepatitis B]
• [Effects of terrestrial input on the harmful algal bloom area--with aluminum as an example]
• [Experimental study on human reaction of determination and performance in virtual environment]
• [Expression of core-binding factor a1 by human skin fibroblasts induced in vitro]
• [Expression of some active peptides and extracellular matrix in the in vitro cultured tissue engineering skin]
• [Investigation of resection and reconstruction procedure of high-sacra tumors]
• [Mechanism of arsenic trioxide-induced apoptosis in hepatic blastoma cells HepG2]
• [Microzooplankton herbivory during red tide-frequent-occurrence period in spring in the East China Sea]
• [Regulative effects and significance of substance P on the expression of basic fibroblast growth factor of granulation tissue fibroblasts in vitro]
• [Role of NADH-cytochrome b(5) reductase in biosynthesis of thyroid hydrogen peroxide]
• [Study of specific targeting cytotoxicity mediated by anti-CD(3)/anti-CD(20) Diabody]
• [Study on gene mutation in 11 Chinese families with Hailey-Hailey disease]
• [Study on making pneumoconiosis model and method of lung lavage in rabbit]
• [Study on the mutation of human telomeric repeat binding factor 1 gene in malignant hematopoietic cell lines]
• [Successful management of sudden respiratory and heart arrest resulting from severe closed thoracoabdominal trauma: report of one case]
• [The expression and mechanism study of BMP in pleomorphic adenoma]
• [The mutation of anti-CD3 antibody (HIT3a) gene and its expression]
• [The prevention of primary liver cancer by selenium in high risk populations]
• kappa-Opioid receptor stimulation contributes to aortic artery dilation through activation of K(ATP) channel in the rats.