The multifunctional protein p54nrb/PSF recruits the exonuclease XRN2 to facilitate pre-mRNA 3' processing and transcription termination.

The multifunctional protein p54nrb/PSF recruits the exonuclease XRN2 to facilitate pre-mRNA 3' processing and transcription termination. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

The multifunctional protein p54nrb/PSF recruits the exonuclease XRN2 to facilitate pre-mRNA 3' processing and transcription termination. Abstract Text:

Syuzo Kaneko,Orit Rozenblatt-Rosen,Matthew Meyerson,James L Manley,

Termination of RNA polymerase II transcription frequently requires a poly(A) signal and cleavage/polyadenylation factors. Recent work has shown that degradation of the downstream cleaved RNA by the exonuclease XRN2 promotes termination, but how XRN2 functions with 3'-processing factors to elicit termination remains unclear. Here we show that XRN2 physically associates with 3'-processing factors and accumulates at the 3' end of a transcribed gene. In vitro 3'-processing assays show that XRN2 is necessary to degrade the downstream RNA, but is not required for 3' cleavage. Significantly, degradation of the 3'-cleaved RNA was stimulated when coupled to cleavage. Unexpectedly, while investigating how XRN2 is recruited to the 3'-processing machinery, we found that XRN2 associates with p54nrb/NonO(p54)-protein-associated splicing factor (PSF), multifunctional proteins involved in several nuclear processes. Strikingly, p54 is also required for degradation of the 3'-cleaved RNA in vitro. p54 is present along the length of genes, and small interfering RNA (siRNA)-mediated knockdown leads to defects in XRN2 recruitment and termination. Together, our data indicate that p54nrb/PSF functions in recruitment of XRN2 to facilitate pre-mRNA 3' processing and transcription termination.

The multifunctional protein p54nrb/PSF recruits the exonuclease XRN2 to facilitate pre-mRNA 3' processing and transcription termination. Publishing Authors By Initials

S Kaneko,O Rozenblatt-Rosen,M Meyerson,JL Manley,

For similar genetic processes: gene expression: transcription, genetic research abstracts see: genetic processes: gene expression: transcription, genetic research

PUBMED ID PMID:

MEDLINE DATE:

The multifunctional protein p54nrb/PSF recruits the exonuclease XRN2 to facilitate pre-mRNA 3' processing and transcription termination. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Genes & development

VOLUME: 21

Page Numbers: 1779-89

Journal Abbreviation: Genes Dev.

ISSN: 0890-9369

DAY: 15

MONTH: Jul

YEAR: 2007

The multifunctional protein p54nrb/PSF recruits the exonuclease XRN2 to facilitate pre-mRNA 3' processing and transcription termination. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8711660

The multifunctional protein p54nrb/PSF recruits the exonuclease XRN2 to facilitate pre-mRNA 3' processing and transcription termination. Keywords Mesh Terms:

KEYWORDS: Transcription, Genetic

MESH TERMS: metabolism

Chemical & Substance for Abstract: The multifunctional protein p54nrb/PSF recruits the exonuclease XRN2 to facilitate pre-mRNA 3' processing and transcription termination. Information

Substance Name: XRN2 protein, human

Registry Number: EC 3.1.13.1

Grant and Affiliation Information for The multifunctional protein p54nrb/PSF recruits the exonuclease XRN2 to facilitate pre-mRNA 3' processing and transcription termination.

AFFILIATION: Department of Biological Sciences, Columbia University, New York, New York 10027, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM28983

ACRONYM: GM

MEDLINETA: Genes Dev

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

The multifunctional protein p54nrb/PSF recruits the exonuclease XRN2 to facilitate pre-mRNA 3' processing and transcription termination Related Publications

• A complex signaling pathway regulates SRp38 phosphorylation and pre-mrna splicing in response to heat shock.
• A ported, proximally-cemented femoral stem for total hip arthroplasty. Development and clinical application.
• Aquaporins: role in cerebral edema and brain water balance.
• Atypical memory phenotype T cells with low homeostatic potential and impaired TCR signaling and regulatory T cell function in Foxn1Delta/Delta mutant mice.
• Concurrent splicing and transcription are not sufficient to enhance splicing efficiency.
• Diabetes mellitus in childhood; report of a local survey.
• Dose-response relationship of mannitol and intracranial pressure: a metaanalysis.
• Estimated average glucose derived from HbA(1c) (eAG): report from European Association for the Study of Diabetes (EASD), Amsterdam 2007.
• Gcm2 is required for the differentiation and survival of parathyroid precursor cells in the parathyroid/thymus primordia.
• Human capping enzyme promotes formation of transcriptional R loops in vitro.
• New approaches to physiological informatics in neurocritical care.
• New insights into mitotic chromosome condensation: a role for the prolyl isomerase pin1.
• Pin1 modulates RNA polymerase II activity during the transcription cycle.
• Prehospital hypoxia affects outcome in patients with traumatic brain injury: a prospective multicenter study.
• Protein-free spliceosomal snRNAs catalyze a reaction that resembles the first step of splicing.
• Relationship between brain tissue oxygen tension and CT perfusion: feasibility and initial results.
• Specific expression of lacZ and cre recombinase in fetal thymic epithelial cells by multiplex gene targeting at the Foxn1 locus.
• Sumoylation modulates the assembly and activity of the pre-mRNA 3' processing complex.
• Sumoylation regulates multiple aspects of mammalian poly(A) polymerase function.
• T cell development from kit-negative progenitors in the foxn1{delta}/{delta} mutant thymus.
• The RNA binding protein RNPS1 alleviates ASF/SF2 depletion-induced genomic instability.
• The impact of smoking-cessation intervention by multiple health professionals.
• The isolation and characterization of glycosaminoglycans in normal human serum.
• The multifunctional protein p54nrb/PSF recruits the exonuclease XRN2 to facilitate pre-mRNA 3' processing and transcription termination.
• The prolyl isomerase Pin1 functions in mitotic chromosome condensation.
• The role of lung function in brain tissue oxygenation following traumatic brain injury.
• The search for alternative splicing regulators: new approaches offer a path to a splicing code.
• Vancomycin use in patients requiring hemodialysis: a literature review.
• Variations in intracellular levels of TATA binding protein can affect specific genes by different mechanisms.
• hnRNP A1 functions with specificity in repression of SMN2 exon 7 splicing.