The Mre11/Rad50/Nbs1 complex limits adeno-associated virus transduction and replication.

The Mre11/Rad50/Nbs1 complex limits adeno-associated virus transduction and replication. Research Abstract Details 

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The Mre11/Rad50/Nbs1 complex limits adeno-associated virus transduction and replication. Abstract Text:

Rachel A Schwartz,Jose Alejandro Palacios,Geoffrey D Cassell,Sarah Adam,Mauro Giacca,Matthew D Weitzman,Rachel A Schwartz,Jose Alejandro Palacios,Geoffrey D Cassell,Sarah Adam,Mauro Giacca,Matthew D Weitzman,

Adeno-associated virus (AAV) is a parvovirus with a small single-stranded DNA genome that relies on cellular replication machinery together with functions supplied by coinfecting helper viruses. The impact of host factors on AAV infection is not well understood. We explored the connection between AAV helper functions supplied by adenovirus and cellular DNA repair proteins. The adenoviral E1b55K/E4orf6 proteins induce degradation of the cellular Mre11 repair complex (MRN) to promote productive adenovirus infection. These viral proteins also augment recombinant AAV transduction and provide crucial helper functions for wild-type AAV replication. Here, we show that MRN poses a barrier to AAV and that the helper function provided by E1b55K/E4orf6 involves MRN degradation. Using a fluorescent method to visualize the viral genome, we show an effect at the viral DNA level. MRN components accumulate at AAV replication centers and recognize the viral inverted terminal repeats. Together, our data suggest that AAV is targeted by MRN and has evolved to exploit adenoviral proteins that degrade these cellular factors.

The Mre11/Rad50/Nbs1 complex limits adeno-associated virus transduction and replication. Publishing Authors By Initials

RA Schwartz,JA Palacios,GD Cassell,S Adam,M Giacca,MD Weitzman,RA Schwartz,JA Palacios,GD Cassell,S Adam,M Giacca,MD Weitzman,

For similar biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: viral physiology: virus replication research abstracts see: biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: viral physiology: virus replication research

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The Mre11/Rad50/Nbs1 complex limits adeno-associated virus transduction and replication. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of virology

VOLUME: 81

Page Numbers: 12936-45

Journal Abbreviation: J. Virol.

ISSN: 1098-5514

DAY: 26

MONTH: 09

YEAR: 2007

The Mre11/Rad50/Nbs1 complex limits adeno-associated virus transduction and replication. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 113724

The Mre11/Rad50/Nbs1 complex limits adeno-associated virus transduction and replication. Keywords Mesh Terms:

KEYWORDS: Virus Replication

MESH TERMS: physiology

Chemical & Substance for Abstract: The Mre11/Rad50/Nbs1 complex limits adeno-associated virus transduction and replication. Information

Substance Name: DNA Repair Enzymes

Registry Number: EC 6.5.1.-

Grant and Affiliation Information for The Mre11/Rad50/Nbs1 complex limits adeno-associated virus transduction and replication.

AFFILIATION: Laboratory of Genetics, Salk Institute for Biological Studies, La Jolla, CA 92037, USA.

Country: United States

AGENCY: United States NCI

GRANT: CA97093

ACRONYM: CA

MEDLINETA: J Virol

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