The molecular signature and cis-regulatory architecture of a C. elegans gustatory neuron.

The molecular signature and cis-regulatory architecture of a C. elegans gustatory neuron. Research Abstract Details 

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The molecular signature and cis-regulatory architecture of a C. elegans gustatory neuron. Abstract Text:

John F Etchberger,Adam Lorch,Monica C Sleumer,Richard Zapf,Steven J Jones,Marco A Marra,Robert A Holt,Donald G Moerman,Oliver Hobert,

Taste receptor cells constitute a highly specialized cell type that perceives and conveys specific sensory information to the brain. The detailed molecular composition of these cells and the mechanisms that program their fate are, in general, poorly understood. We have generated serial analysis of gene expression (SAGE) libraries from two distinct populations of single, isolated sensory neuron classes, the gustatory neuron class ASE and the thermosensory neuron class AFD, from the nematode Caenorhabditis elegans. By comparing these two libraries, we have identified >1000 genes that define the ASE gustatory neuron class on a molecular level. This set of genes contains determinants of the differentiated state of the ASE neuron, such as a surprisingly complex repertoire of transcription factors (TFs), ion channels, neurotransmitters, and receptors, as well as seven-transmembrane receptor (7TMR)-type putative gustatory receptor genes. Through the in vivo dissection of the cis-regulatory regions of several ASE-expressed genes, we identified a small cis-regulatory motif, the "ASE motif," that is required for the expression of many ASE-expressed genes. We demonstrate that the ASE motif is a binding site for the C2H2 zinc finger TF CHE-1, which is essential for the correct differentiation of the ASE gustatory neuron. Taken together, our results provide a unique view of the molecular landscape of a single neuron type and reveal an important aspect of the regulatory logic for gustatory neuron specification in C. elegans.

The molecular signature and cis-regulatory architecture of a C. elegans gustatory neuron. Publishing Authors By Initials

JF Etchberger,A Lorch,MC Sleumer,R Zapf,SJ Jones,MA Marra,RA Holt,DG Moerman,O Hobert,

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The molecular signature and cis-regulatory architecture of a C. elegans gustatory neuron. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Genes & development

VOLUME: 21

Page Numbers: 1653-74

Journal Abbreviation: Genes Dev.

ISSN: 0890-9369

DAY: 1

MONTH: Jul

YEAR: 2007

The molecular signature and cis-regulatory architecture of a C. elegans gustatory neuron. Information

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LANGUAGE: eng

NlmUniqueID: 8711660

The molecular signature and cis-regulatory architecture of a C. elegans gustatory neuron. Keywords Mesh Terms:

KEYWORDS: Zinc Fingers

MESH TERMS: physiology

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Substance Name: Transcription Factors

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Grant and Affiliation Information for The molecular signature and cis-regulatory architecture of a C. elegans gustatory neuron.

AFFILIATION: Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University Medical Center, New York, NY 10032, USA.

Country: United States

AGENCY: United States NINDS

GRANT: R01 NS050266-01

ACRONYM: NS

MEDLINETA: Genes Dev

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