The maturation of mucus-secreting gastric epithelial progenitors into digestive-enzyme secreting zymogenic cells requires Mist1.

The maturation of mucus-secreting gastric epithelial progenitors into digestive-enzyme secreting zymogenic cells requires Mist1. Research Abstract Details 

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The maturation of mucus-secreting gastric epithelial progenitors into digestive-enzyme secreting zymogenic cells requires Mist1. Abstract Text:

Victoria G Ramsey,Jason M Doherty,Christopher C Chen,Thaddeus S Stappenbeck,Stephen F Konieczny,Jason C Mills,

Continuous regeneration of digestive enzyme (zymogen)-secreting chief cells is a normal aspect of stomach function that is disrupted in precancerous lesions (e.g. metaplasias, chronic atrophy). The cellular and genetic pathways that underlie zymogenic cell (ZC) differentiation are poorly understood. Here, we describe a gene expression analysis of laser capture microdissection purified gastric cell populations that identified the bHLH transcription factor Mist1 as a potential ZC regulatory factor. Our molecular and ultrastructural analysis of proliferation, migration and differentiation of the gastric unit in Mist1(-/-) and control mice supports a model whereby wild-type ZC progenitors arise as neck cells in the proliferative (isthmal) zone of the gastric unit and become transitional cells (TCs) with molecular and ultrastructural characteristics of both enzyme-secreting ZCs and mucus-secreting neck cells as they migrate to the neck-base zone interface. Thereafter, they rapidly differentiate into mature ZCs as they enter the base. By contrast, Mist1(-/-) neck cells differentiate normally, but ZCs in the mature, basal portion of the gastric unit uniformly exhibit multiple apical cytoplasmic structural abnormalities. This defect in terminal ZC differentiation is also associated with markedly increased abundance of TCs, especially in late-stage TCs that predominantly have features of immature ZCs. Thus, we present an in vivo system for analysis of ZC differentiation, present molecular evidence that ZCs differentiate from neck cell progenitors and identify Mist1 as the first gene with a role in this clinically important process.

The maturation of mucus-secreting gastric epithelial progenitors into digestive-enzyme secreting zymogenic cells requires Mist1. Publishing Authors By Initials

VG Ramsey,JM Doherty,CC Chen,TS Stappenbeck,SF Konieczny,JC Mills,

For similar digestive system: gastrointestinal tract: upper gastrointestinal tract: stomach research abstracts see: digestive system: gastrointestinal tract: upper gastrointestinal tract: stomach research

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The maturation of mucus-secreting gastric epithelial progenitors into digestive-enzyme secreting zymogenic cells requires Mist1. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Development (Cambridge, England)

VOLUME: 134

Page Numbers: 211-22

Journal Abbreviation: Development

ISSN: 0950-1991

DAY: 3

MONTH: Jan

YEAR: 2007

The maturation of mucus-secreting gastric epithelial progenitors into digestive-enzyme secreting zymogenic cells requires Mist1. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8701744

The maturation of mucus-secreting gastric epithelial progenitors into digestive-enzyme secreting zymogenic cells requires Mist1. Keywords Mesh Terms:

KEYWORDS: Stomach

MESH TERMS: physiology

Chemical & Substance for Abstract: The maturation of mucus-secreting gastric epithelial progenitors into digestive-enzyme secreting zymogenic cells requires Mist1. Information

Substance Name: Mist1 protein, mouse

Registry Number: 0

Grant and Affiliation Information for The maturation of mucus-secreting gastric epithelial progenitors into digestive-enzyme secreting zymogenic cells requires Mist1.

AFFILIATION: Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO 63110, USA.

Country: England

AGENCY: United States NIDDK

GRANT: R01 DK55489

ACRONYM: DK

MEDLINETA: Development

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