The locus control region activates serpin gene expression through recruitment of liver-specific transcription factors and RNA polymerase II.

The locus control region activates serpin gene expression through recruitment of liver-specific transcription factors and RNA polymerase II. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

The locus control region activates serpin gene expression through recruitment of liver-specific transcription factors and RNA polymerase II. Abstract Text:

Hui Zhao,Richard D Friedman,R E K Fournier,

The human serine protease inhibitor (serpin) gene cluster at 14q32.1 comprises 11 serpin genes, many of which are expressed specifically in hepatic cells. Previous studies identified a locus control region (LCR) upstream of the human alpha1-antitrypsin (alpha1AT) gene that is required for gene activation, chromatin remodeling, and histone acetylation throughout the proximal serpin subcluster. Here we show that the LCR interacts with multiple liver-specific transcription factors, including hepatocyte nuclear factor 3beta (HNF-3beta), HNF-6alpha, CCAAT/enhancer binding protein alpha (C/EBPalpha), and C/EBPbeta. RNA polymerase II is also recruited to the locus through the LCR. Nongenic transcription at both the LCR and an upstream regulatory region was detected, but the deletion of the LCR abolished transcription at both sites. The deletion of HNF-3 and HNF-6 binding sites within the LCR reduced histone acetylation at both the LCR and the upstream regulatory region and decreased the transcription of the alpha1AT, corticosteroid binding globulin, and protein Z-dependent protease inhibitor genes. These results suggest that the LCR activates genes in the proximal serpin subcluster by recruiting liver-specific transcription factors and components of the general transcription machinery to regulatory regions upstream of the alpha1AT gene.

The locus control region activates serpin gene expression through recruitment of liver-specific transcription factors and RNA polymerase II. Publishing Authors By Initials

H Zhao,RD Friedman,RE Fournier,

For similar peptides: proteinase inhibitory proteins, secretory: alpha 1-antitrypsin research abstracts see: peptides: proteinase inhibitory proteins, secretory: alpha 1-antitrypsin research

PUBMED ID PMID:

MEDLINE DATE:

The locus control region activates serpin gene expression through recruitment of liver-specific transcription factors and RNA polymerase II. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Molecular and cellular biology

VOLUME: 27

Page Numbers: 5286-95

Journal Abbreviation: Mol. Cell. Biol.

ISSN: 0270-7306

DAY: 25

MONTH: 05

YEAR: 2007

The locus control region activates serpin gene expression through recruitment of liver-specific transcription factors and RNA polymerase II. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8109087

The locus control region activates serpin gene expression through recruitment of liver-specific transcription factors and RNA polymerase II. Keywords Mesh Terms:

KEYWORDS: alpha 1-Antitrypsin

MESH TERMS: genetics

Chemical & Substance for Abstract: The locus control region activates serpin gene expression through recruitment of liver-specific transcription factors and RNA polymerase II. Information

Substance Name: RNA Polymerase II

Registry Number: EC 2.7.7.-

Grant and Affiliation Information for The locus control region activates serpin gene expression through recruitment of liver-specific transcription factors and RNA polymerase II.

AFFILIATION: Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N., Seattle, WA 98109, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM26449

ACRONYM: GM

MEDLINETA: Mol Cell Biol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

The locus control region activates serpin gene expression through recruitment of liver-specific transcription factors and RNA polymerase II Related Publications

• A 10-s sprint performed prior to moderate-intensity exercise prevents early post-exercise fall in glycaemia in individuals with type 1 diabetes.
• Changes in hydraulic conductivity, mechanical properties, and density reflecting the fall in strain along the lateral roots of two species of tropical trees.
• Functional results of unilateral lung volume reduction surgery in alpha1-antitrypsin deficient patients.
• Genetic diversity of Bartonella henselae in human infection detected with multispacer typing.
• Host mediated anti-tumor effect of oncolytic Newcastle disease virus after locoregional application.
• Identifying effects of snoRNA-guided modifications on the synthesis and function of the yeast ribosome.
• Introducing the external link model for studying spine fixation and misalignment: part 1--need, rationale, and applications.
• Introducing the external link model for studying spine fixation and misalignment: part 2, Biomechanical features.
• New bioinformatic tools for analysis of nucleotide modifications in eukaryotic rRNA.
• New developments in MALDI imaging for pathology proteomic studies.
• New insights into managing the risk of hypoglycaemia associated with intermittent high-intensity exercise in individuals with type 1 diabetes mellitus : implications for existing guidelines.
• Protein expression from synthetic genes: selection of clones using GFP.
• Reappraisal of the comparative cost of human locomotion using gait-specific allometric analyses.
• Sorption of aroma compounds in PET and PVC during the storage of a strawberry syrup.
• Specific MALDI imaging and profiling for biomarker hunting and validation: fragment of the 11S proteasome activator complex, Reg alpha fragment, is a new potential ovary cancer biomarker.
• Study of solid-phase synthesis and purification strategies for the preparation of polyglutamine peptides.
• The U1 snRNA hairpin II as a RNA affinity tag for selecting snoRNP complexes.
• The locus control region activates serpin gene expression through recruitment of liver-specific transcription factors and RNA polymerase II.
• Veno-occlusive disease of the liver after lung transplantation.
• [Asbestos in France]
• [Diuretic-based therapy]
• [Not Available.]
• [Not Available.]
• [Not Available.]
• [Not Available.]
• [Not Available.]
• [Not Available.]
• [Not Available.]
• [Not Available.]
• rRNA Modifications in an Intersubunit Bridge of the Ribosome Strongly Affect Both Ribosome Biogenesis and Activity.