The Kaposi's sarcoma-associated herpesvirus K5 E3 ubiquitin ligase modulates targets by multiple molecular mechanisms.

The Kaposi's sarcoma-associated herpesvirus K5 E3 ubiquitin ligase modulates targets by multiple molecular mechanisms. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

The Kaposi's sarcoma-associated herpesvirus K5 E3 ubiquitin ligase modulates targets by multiple molecular mechanisms. Abstract Text:

Robert E Means,Sabine M Lang,Jae U Jung,

Kaposi's sarcoma-associated herpesvirus encodes two highly related membrane-associated, RING-CH-containing (MARCH) family E3 ubiquitin ligases, K3 and K5, that can down regulate a variety of cell surface proteins through enhancement of their endocytosis and degradation. In this report we present data that while K5 modulation of major histocompatibility complex class I (MHC-I) closely mirrors the mechanisms used by K3, alternative molecular pathways are utilized by this E3 ligase in the down regulation of intercellular adhesion molecule 1 (ICAM-1) and B7.2. Internalization assays demonstrate that down regulation of each target can occur through increased endocytosis from the cell surface. However, mutation of a conserved tyrosine-based endocytosis motif in K5 resulted in a protein lacking the ability to direct an increased rate of MHC-I or ICAM-1 internalization but still able to down regulate B7.2 in a ubiquitin-dependent but endocytosis-independent manner. Further, mutation of two acidic clusters abolished K5-mediated MHC-I degradation while only slightly decreasing ICAM-1 or B7.2 protein destruction. This same mutant abolished detectable ubiquitylation of all targets. These data indicate that while K5 can act as an E3 ubiquitin ligase to directly mediate cell surface molecule destruction, regulation of its targets occurs through multiple pathways, including ubiquitin-independent mechanisms.

The Kaposi's sarcoma-associated herpesvirus K5 E3 ubiquitin ligase modulates targets by multiple molecular mechanisms. Publishing Authors By Initials

RE Means,SM Lang,JU Jung,

For similar enzymes and coenzymes: enzymes: ligases: ubiquitin-protein ligase complexes: ubiquitin-protein ligases research abstracts see: enzymes and coenzymes: enzymes: ligases: ubiquitin-protein ligase complexes: ubiquitin-protein ligases research

PUBMED ID PMID:

MEDLINE DATE:

The Kaposi's sarcoma-associated herpesvirus K5 E3 ubiquitin ligase modulates targets by multiple molecular mechanisms. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of virology

VOLUME: 81

Page Numbers: 6573-83

Journal Abbreviation: J. Virol.

ISSN: 0022-538X

DAY: 4

MONTH: 04

YEAR: 2007

The Kaposi's sarcoma-associated herpesvirus K5 E3 ubiquitin ligase modulates targets by multiple molecular mechanisms. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 113724

The Kaposi's sarcoma-associated herpesvirus K5 E3 ubiquitin ligase modulates targets by multiple molecular mechanisms. Keywords Mesh Terms:

KEYWORDS: Ubiquitin-Protein Ligases

MESH TERMS: chemistry

Chemical & Substance for Abstract: The Kaposi's sarcoma-associated herpesvirus K5 E3 ubiquitin ligase modulates targets by multiple molecular mechanisms. Information

Substance Name: Ubiquitin-Protein Ligases

Registry Number: EC 6.3.2.19

Grant and Affiliation Information for The Kaposi's sarcoma-associated herpesvirus K5 E3 ubiquitin ligase modulates targets by multiple molecular mechanisms.

AFFILIATION: Department of Pathology, Yale University School of Medicine, P.O. Box 208023, New Haven, CT 06520, USA. Robert.Means@Yale.edu

Country: United States

AGENCY: United States NCI

GRANT: R21 CA102535

ACRONYM: CA

MEDLINETA: J Virol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

The Kaposi's sarcoma-associated herpesvirus K5 E3 ubiquitin ligase modulates targets by multiple molecular mechanisms Related Publications

• (-)-Epigallocatechin-3-gallate inhibits monocyte chemotactic protein-1 expression in endothelial cells via blocking NF-kappaB signaling.
• Bayesian analysis of paired survival data using a bivariate exponential distribution.
• Bilateral chylothoraces without chyle leakage after left-sided neck dissection for thyroid cancer: report of two cases.
• Broadband noise reduction of piezoelectric smart panel featuring negative-capacitive-converter shunt circuit.
• Complete mitochondrial genome of the rabbitfish Siganus fuscescens (Perciformes, Siganidae).
• Developmental properties of the Hertwig's epithelial root sheath in mice.
• Downregulation of gamma interferon receptor 1 by Kaposi's sarcoma-associated herpesvirus K3 and K5.
• E1 component of pyruvate dehydrogenase complex does not regulate the expression of NADPH-ferredoxin reductase in Azotobacter vinelandii.
• Effect of direct-fed microbials on performance, diet digestibility, and rumen characteristics of Holstein dairy cows.
• Effects of pioglitazone and insulin on tight glycaemic control assessed by the continuous glucose monitoring system : a monocentric, parallel-cohort study.
• Efficacy of low-dose tamsulosin on lower urinary tract symptoms suggestive of benign prostatic hyperplasia : a nonblind multicentre korean study.
• Elevated plasma osteopontin as marker for distant metastases and poor survival in patients with renal cell carcinoma.
• Free radical scavenging and skin penetration of troxerutin and vitamin derivatives.
• Gene expression profiling in lung tissues from rats exposed to formaldehyde.
• Germline methylation patterns inferred from local nucleotide frequency of repetitive sequences in the human genome.
• Inhibition of interferon regulatory factor 7 (IRF7)-mediated interferon signal transduction by the Kaposi's sarcoma-associated herpesvirus viral IRF homolog vIRF3.
• Learning-induced enduring changes in functional connectivity among prefrontal cortical neurons.
• Monitoring Driver's Alertness Based on the Driving Performance Estimation and the EEG Power Spectrum Analysis.
• Performance of membrane-coupled organic acid fermentor for the resources recovery form municipal sewage sludge.
• Prognostic significance of the MUC1 and MUC4 expressions in thyroid papillary carcinoma.
• Reliable WAnT time about competitive abilities performed by elite racing cyclists in Korea: 2404: Board #83 June 1 2:00 PM - 3:30 PM.
• Statistics for proteomics: a review of tools for analyzing experimental data.
• The Kaposi's sarcoma-associated herpesvirus K5 E3 ubiquitin ligase modulates targets by multiple molecular mechanisms.
• The nucleoside analog sangivamycin induces apoptotic cell death in breast carcinoma MCF7/adriamycin-resistant cells via protein kinase Cdelta and JNK activation.
• Total knee arthroplasty in patients with severe deformities due to rheumatoid arthritis.
• Total knee arthroplasty in patients with severe deformities due to rheumatoid arthritis.
• [Analysis of survival and factors affecting the survival after surgical resection of peripheral cholangiocarcinoma: 318 cases in single institute]
• [Developmental direction for review system of the journal of korean academy of nursing]
• [Nursing images appearing in elementary school textbooks under the 7th national curriculum]
• [The officers of health in the department of Moselle.]