The involvement of secondary signaling molecules in cytochrome P-450 1A1-mediated inducible nitric oxide synthase expression in benzo(a)pyrene-treated rat polymorphonuclear leukocytes.

The involvement of secondary signaling molecules in cytochrome P-450 1A1-mediated inducible nitric oxide synthase expression in benzo(a)pyrene-treated rat polymorphonuclear leukocytes. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

The involvement of secondary signaling molecules in cytochrome P-450 1A1-mediated inducible nitric oxide synthase expression in benzo(a)pyrene-treated rat polymorphonuclear leukocytes. Abstract Text:

Abhai Kumar,Ghanshyam Upadhyay,Dinesh Raj Modi,Mahendra Pratap Singh,Abhai Kumar,Ghanshyam Upadhyay,Dinesh Raj Modi,Mahendra Pratap Singh,Abhai Kumar,Ghanshyam Upadhyay,Dinesh Raj Modi,Mahendra Pratap Singh,

Benzo(a)pyrene induces cytochrome P-450 1A1 (CYP1A1) expression in rat polymorphonuclear leukocytes (PMNs) that upregulates expression of inducible nitric oxide synthase (iNOS). In the present study, the involvement of secondary signaling molecules in CYP1A1-mediated augmentation of iNOS expression in benzo(a)pyrene-treated rat PMNs was investigated. PMNs were isolated from the peripheral blood of controls and benzo(a)pyrene-treated rats. The expression and/or activity of CYP1A1, iNOS, tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1beta), and intracellular calcium ([Ca(2+)]i) concentrations were measured in control and benzo(a)pyrene-treated rat PMNs with and without alpha-naphthoflavone, aminoguanidine, genistein, pyrrolidine dithiocarbamate (PDTC), felodipine, or SB202190 pre-treatment. A significant elevation in CYP1A1 and [Ca(2+)]i was observed in benzo(a)pyrene-treated rat PMNs, which was significantly restored by alpha-naphthoflavone or genistein. Neither PDTC, SB202190, nor aminoguanidine altered the benzo(a)pyrene-mediated increase in [Ca(2+)]i. Although felodipine reduced the benzo(a)pyrene-mediated increase in [Ca(2+)]i, no significant change was observed in CYP1A1 expression and activity. Benzo(a)pyrene-augmented iNOS expression and activity in PMNs were significantly reverse by felodipine, genistein, or PDTC. Benzo(a)pyrene also induced TNF-alpha and IL-1beta production in PMNs, which was significantly reversed by genistein. The results demonstrated the involvement of [Ca(2+)]i, tyrosine kinase, inflammatory cytokines, and NF-kappaB in CYP1A1-mediated iNOS expression in benzo(a)pyrene-treated rat PMNs.

The involvement of secondary signaling molecules in cytochrome P-450 1A1-mediated inducible nitric oxide synthase expression in benzo(a)pyrene-treated rat polymorphonuclear leukocytes. Publishing Authors By Initials

A Kumar,G Upadhyay,DR Modi,MP Singh,A Kumar,G Upadhyay,DR Modi,MP Singh,A Kumar,G Upadhyay,DR Modi,MP Singh,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

The involvement of secondary signaling molecules in cytochrome P-450 1A1-mediated inducible nitric oxide synthase expression in benzo(a)pyrene-treated rat polymorphonuclear leukocytes. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Life sciences

VOLUME: 81

Page Numbers: 1575-84

Journal Abbreviation: Life Sci.

ISSN: 0024-3205

DAY: 10

MONTH: 10

YEAR: 2007

The involvement of secondary signaling molecules in cytochrome P-450 1A1-mediated inducible nitric oxide synthase expression in benzo(a)pyrene-treated rat polymorphonuclear leukocytes. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 375521

The involvement of secondary signaling molecules in cytochrome P-450 1A1-mediated inducible nitric oxide synthase expression in benzo(a)pyrene-treated rat polymorphonuclear leukocytes. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: The involvement of secondary signaling molecules in cytochrome P-450 1A1-mediated inducible nitric oxide synthase expression in benzo(a)pyrene-treated rat polymorphonuclear leukocytes. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for The involvement of secondary signaling molecules in cytochrome P-450 1A1-mediated inducible nitric oxide synthase expression in benzo(a)pyrene-treated rat polymorphonuclear leukocytes.

AFFILIATION: Environmental Biotechnology Division, Industrial Toxicology Research Centre (ITRC), Lucknow-226 001, India.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Life Sci

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

The involvement of secondary signaling molecules in cytochrome P-450 1A1-mediated inducible nitric oxide synthase expression in benzoapyrene-treated rat polymorphonuclear leukocytes Related Publications

• A definition of the breeding season of Pool Dorset ewes.
• A shift from significance test to hypothesis test through power analysis in medical research.
• Bio-histopathological studies of endometrium in repeat breeding buffaloes (Bubalus bubalis).
• Challenging and Changing Gender Attitudes among Young Men in Mumbai, India.
• Diallyl trisulfide inhibits angiogenic features of human umbilical vein endothelial cells by causing Akt inactivation and down-regulation of VEGF and VEGF-R2.
• ERK/MAPK pathway regulates GABAA receptors.
• Effect of adenotonsillitis on peak exploratory flow rate in children.
• Effects of bull and heparin and sperm concentrations on in vitro fertilization of buffalo (Bubalus bubalis ) oocytes matured in vitro.
• Efficacy of the Hamilton Thorn Motility Analyzer (HTM-2030) for the evaluation of bovine semen.
• Embryo transfer as a means of controlling the transmission of viral infections. XIII. Failure to transmit foot-and-mouth disease virus through the transfer of embryos from viremic donors.
• Expression of the cytoplasmic domain of beta1 integrin induces apoptosis in adult rat ventricular myocytes (ARVM) via the involvement of caspase-8 and mitochondrial death pathway.
• GSNO attenuates EAE disease by S-nitrosylation-mediated modulation of endothelial-monocyte interactions.
• How much canola or cottonseed meals can be used for commercial chicken meat production.
• In patients with mild hypertension, does exercise and a gradual rather than abrupt increase in fatty acid and salt intake cause less rise in cardiovascular risk factors?
• In vitro maturation and fertilization of buffalo oocytes (Bubalus bubalis ): Effects of media, hormones and sera.
• Integrated preconcentration SDS-PAGE of proteins in microchips using photopatterned cross-linked polyacrylamide gels.
• Interaction of maternal protein and carbon monoxide on pup mortality in mice: implications for global infant mortality.
• Mitochondria as targets for detection and treatment of cancer.
• On-chip native gel electrophoresis-based immunoassays for tetanus antibody and toxin.
• Phenethyl isothiocyanate-induced apoptosis in PC-3 human prostate cancer cells is mediated by reactive oxygen species-dependent disruption of the mitochondrial membrane potential.
• Plasma alpha-tocopherol profiles in sheep after oral administration of dl-alpha-tocopheryl acetate and d-alpha-tocopheryl succinate.
• Prophylactic gabapentin for prevention of postoperative nausea and vomiting in patients undergoing laparoscopic cholecystectomy: A randomized, double-blind, placebo-controlled study.
• Proteomic analyses of murine macrophages treated with Bacillus anthracis lethal toxin.
• Proteomic analysis of mitochondria-to-nucleus retrograde response in human cancer.
• Responses of different doses of prostaglandin F(2) alpha on estrus induction, fertility and progesterone levels in subestrous buffaloes.
• Seasonal variation in freezability of buffalo semen.
• Steroid receptor coactivator AIB1 in endometrial carcinoma, hyperplasia and normal endometrium: Correlation with clinicopathologic parameters and biomarkers.
• The mental health challenge in Sri Lanka from working within the disaster area.
• Up-regulation of transient receptor potential canonical 1 (TRPC1) following sarco(endo)plasmic reticulum Ca2+ ATPase 2 gene silencing promotes cell survival: a potential role for TRPC1 in Darier's disease.
• Variation in angiotensin-converting enzyme activity in the testes of prepubertal, pubertal and adult cockerels.