The interplay between calcium and the in vitro lectin and chaperone activities of calreticulin.

The interplay between calcium and the in vitro lectin and chaperone activities of calreticulin. Research Abstract Details 

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The interplay between calcium and the in vitro lectin and chaperone activities of calreticulin. Abstract Text:

Ianina L Conte,Natasha Keith,Clara ,Armando J Parodi,Julio J Caramelo,

The ER resident protein calreticulin fulfills at least two important roles. It works as a chaperone preventing Golgi exit of non-native protein species and enhancing protein folding efficiency in either N-glycan-dependent, lectin chaperone, or classical chaperone, N-glycan-independent, modes and is one of the main calcium buffers in the cell. This last feature is independent from the lectin chaperone properties of the protein as this last activity is also observed in a CRT fragment lacking calcium buffer capacity. Here we study the interplay between calcium and the lectin and chaperone activities of CRT. The affinity of CRT for monoglucosylated glycans measured in solution by equilibrium dialysis and fluorescence anisotropy was not affected by the absence of calcium. Binding of CRT to monoglucosylated neoglycoproteins displaying either native or molten globule-like conformations was also independent of calcium concentration. Moreover, calcium and monoglucosylated glycans stabilized the CRT structure in an apparent additive, independent manner when the protein was subjected to thermal or urea-induced denaturation. In addition, the ability of CRT to decrease the level of aggregation of a chemically denatured monoglucosylated and nonglycosylated protein was also independent of calcium level.

The interplay between calcium and the in vitro lectin and chaperone activities of calreticulin. Publishing Authors By Initials

IL Conte,N Keith,C ,AJ Parodi,JJ Caramelo,

For similar organic chemicals: urea research abstracts see: organic chemicals: urea research

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The interplay between calcium and the in vitro lectin and chaperone activities of calreticulin. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biochemistry

VOLUME: 46

Page Numbers: 4671-80

Journal Abbreviation:

ISSN: 0006-2960

DAY: 27

MONTH: 03

YEAR: 2007

The interplay between calcium and the in vitro lectin and chaperone activities of calreticulin. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370623

The interplay between calcium and the in vitro lectin and chaperone activities of calreticulin. Keywords Mesh Terms:

KEYWORDS: Urea

MESH TERMS: pharmacology

Chemical & Substance for Abstract: The interplay between calcium and the in vitro lectin and chaperone activities of calreticulin. Information

Substance Name: Calcium

Registry Number: 7440-70-2

Grant and Affiliation Information for The interplay between calcium and the in vitro lectin and chaperone activities of calreticulin.

AFFILIATION: Laboratory of Glycobiology, Fundación Instituto Leloir, Av. Patricias Argentinas 435, Buenos Aires C1405BWE, Argentina.

Country: United States

AGENCY: United States NIGMS

GRANT: GM44500

ACRONYM: GM

MEDLINETA: Biochemistry

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