[The inductive activation of the promoter of pib gene]

[The inductive activation of the promoter of pib gene] Research Abstract Details 

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[The inductive activation of the promoter of pib gene] Abstract Text:

Chan-Juan Li,Shi-Hu Yang,Liang Wu,Jian-Min Wan,

A 5.7 kb putative promoter region of pib gene was isolated from the pib genomic clone and substituted for the 35S promoter upstream of gus gene in plasmid pCAMBIA1301 to construct a new plant expression vector pNAR604 (putative pib promoter-GUS + 35S-hpt). From Agrobacterium-mediated transformation and hygromycin selective culture in vitro, hygromycin resistant calli and 36 transgenic rice (Oryza sativa L.) plants were obtained. Histochemical assays of GUS activity showed that no expression was observed in the resistant calli and roots from transgenic rice if cultured under light, but after 24 h dark treatment there was strong GUS staining. Fluorimetric quantitative analysis indicated that GUS expression was organ-specific in transgenic rice. Without the dark treatment, GUS activity in roots and stems were about 7 and 3 times higher than in leaves in which GUS activity was only trace detected. After 24 h dark treatment, GUS activity in roots, stems and leaves of transgenic plants were all promoted and the largest increase was observed in leaves. Twenty-four hours after spraying with 5 mmol/L SA (Salicylic Acid) or 0.3 mol/L NaCl, GUS activity in leaves of the transgenic plants was 2.7 or 3.6 times respectively higher than untreated control. It was confirmed that an inductive promoter was involved in this 5.7 kb upstream region of pib gene, and dark, SA and NaCl treatments were inductive factors for pib promoter.

[The inductive activation of the promoter of pib gene] Publishing Authors By Initials

CJ Li,SH Yang,L Wu,JM Wan,

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[The inductive activation of the promoter of pib gene] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Yi chuan = Hereditas / Zhongguo yi chuan xue hui b

VOLUME: 28

Page Numbers: 689-94

Journal Abbreviation: Yi Chuan

ISSN: 0253-9772

DAY: 4

MONTH: Jun

YEAR: 2006

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LANGUAGE: chi

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AFFILIATION: National Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Center, Nanjing Agricultural University, Nanjing 210095, China.

Country: China

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MEDLINETA: Yi Chuan

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