The implications of proteolytic background for shotgun proteomics.

The implications of proteolytic background for shotgun proteomics. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

The implications of proteolytic background for shotgun proteomics. Abstract Text:

Paola Picotti,Ruedi Aebersold,Bruno Domon,

The analysis by liquid chromatography coupled to tandem mass spectrometry of complex peptide mixtures, generated by proteolysis of protein samples, is the main proteomics method used today. The approach is based on the assumption that each protein present in a sample reproducibly and predictably generates a relatively small number of peptides that can be identified by mass spectrometry. In this study this assumption was examined by a targeted peptide sequencing strategy using inclusion lists to trigger peptide fragmentation attempts. It was found that the number of peptides observed from a single protein is at least one order of magnitude greater than previously assumed. This unexpected complexity of proteomics samples implies substantial technical challenges, explains some perplexing results in the proteomics literature, and prompts the need for developing alternative experimental strategies for the rapid and comprehensive analysis of proteomes.

The implications of proteolytic background for shotgun proteomics. Publishing Authors By Initials

P Picotti,R Aebersold,B Domon,

For similar enzymes and coenzymes: enzymes: hydrolases: peptide hydrolases: endopeptidases: serine endopeptidases: trypsin research abstracts see: enzymes and coenzymes: enzymes: hydrolases: peptide hydrolases: endopeptidases: serine endopeptidases: trypsin research

PUBMED ID PMID:

MEDLINE DATE:

The implications of proteolytic background for shotgun proteomics. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecular & cellular proteomics : MCP

VOLUME: 6

Page Numbers: 1589-98

Journal Abbreviation: Mol. Cell Proteomics

ISSN: 1535-9476

DAY: 28

MONTH: 05

YEAR: 2007

The implications of proteolytic background for shotgun proteomics. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101125647

The implications of proteolytic background for shotgun proteomics. Keywords Mesh Terms:

KEYWORDS: Trypsin

MESH TERMS: chemistry

Chemical & Substance for Abstract: The implications of proteolytic background for shotgun proteomics. Information

Substance Name: Trypsin

Registry Number: EC 3.4.21.4

Grant and Affiliation Information for The implications of proteolytic background for shotgun proteomics.

AFFILIATION: Institute of Molecular Systems Biology, ETH Zürich, CH-8093, Zürich, Switzerland.

Country: United States

AGENCY: United States NHLBI

GRANT: N01 HV28179

ACRONYM: HV

MEDLINETA: Mol Cell Proteomics

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

The implications of proteolytic background for shotgun proteomics Related Publications

• High sensitivity detection of plasma proteins by multiple reaction monitoring of N-glycosites.
• Managing abandoned farmland: the need to link biological and sociological aspects.
• The implications of proteolytic background for shotgun proteomics.