The immunohistochemical localization of Fas and Fas ligand in jaw bone and tooth germ of human fetuses.

The immunohistochemical localization of Fas and Fas ligand in jaw bone and tooth germ of human fetuses. Research Abstract Details 

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The immunohistochemical localization of Fas and Fas ligand in jaw bone and tooth germ of human fetuses. Abstract Text:

S Hatakeyama,N Tomichi,Y Ohara-Nemoto,M Satoh,

The cellular localization and roles of bone morphogenetic protein (BMP)-2 and apoptosis-associating factors in human orofacial development remain unclear. In this study, BMP-2, osteocalcin, and TGF-beta, which are bone-differentiating markers, apoptosis-associating factors (i.e., Bcl-2, Bax, Fas, and Fas ligand), apoptotic cells detected by the in situ 3'-end labeling method (TUNEL), and proliferating cell nuclear antigen (PCNA) were immunohistochemically examined in the heads (in particular, the jaw bone and tooth germs) of human fetuses of 11-week pregnancy. BMP-2 was positive in osteoblasts and newly formed osteoid of the incisive and palatal bone of the maxilla and the mandible, which indicated that BMP-2 was exclusively involved in intramembranous ossification in the human fetal head. Fas was positive in the cytoplasm of osteocytes and a few osteoblasts. In contrast, Fas ligand was positive in the cytoplasm of osteoblasts and abundant in the stroma of the osteoblastic layer, periosteum, and perichondrium. The Fas ligand in the stroma was recognized as the soluble form, which was possibly produced by osteoblasts. TUNEL-positive apoptotic cells were found in a few osteocytes and a few osteoblastic cells in new bone, and in monocytes of degenerate Meckel's cartilage. The induction of apoptosis observed in monocytes seems to be caused via a Fas-Fas ligand cell death system, because some of these monocytes were Fas-positive, and most of them were Fas ligand-positive. Interestingly, the abundant soluble Fas ligand observed in the periosteum probably protects the bone-formative zone from the invasion of the activated lymphocytes by binding to Fas expressing in these lymphocytes and killing these cells. Fas and Fas ligand were focally positive in the dental lamina and inner enamel epithelium and cusps of the enamel organ, nevertheless, the presence of TUNEL-positive cells was very rare. Bcl-2 was clearly and Bax was weakly positive in the cells throughout the dental lamina and enamel organ. These findings indicated that Fas-mediated apoptosis was inhibited by the Bcl-2 family in the development of teeth.

The immunohistochemical localization of Fas and Fas ligand in jaw bone and tooth germ of human fetuses. Publishing Authors By Initials

S Hatakeyama,N Tomichi,Y Ohara-Nemoto,M Satoh,

For similar peptides: intracellular signaling peptides and proteins: apoptosis regulatory proteins: proto-oncogene proteins c-bcl-2: bcl-2-associated x protein research abstracts see: peptides: intracellular signaling peptides and proteins: apoptosis regulatory proteins: proto-oncogene proteins c-bcl-2: bcl-2-associated x protein research

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The immunohistochemical localization of Fas and Fas ligand in jaw bone and tooth germ of human fetuses. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Calcified tissue international

VOLUME: 66

Page Numbers: 330-7

Journal Abbreviation: Calcif. Tissue Int.

ISSN: 0171-967X

DAY: 19

MONTH: May

YEAR: 2000

The immunohistochemical localization of Fas and Fas ligand in jaw bone and tooth germ of human fetuses. Information

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LANGUAGE: eng

NlmUniqueID: 7905481

The immunohistochemical localization of Fas and Fas ligand in jaw bone and tooth germ of human fetuses. Keywords Mesh Terms:

KEYWORDS: bcl-2-Associated X Protein

MESH TERMS: metabolism

Chemical & Substance for Abstract: The immunohistochemical localization of Fas and Fas ligand in jaw bone and tooth germ of human fetuses. Information

Substance Name: Osteocalcin

Registry Number: 104982-03-8

Grant and Affiliation Information for The immunohistochemical localization of Fas and Fas ligand in jaw bone and tooth germ of human fetuses.

AFFILIATION: Department of Oral Pathology, School of Dentistry, Iwate Medical University, 19-1 Uchimaru, Morioka, 020-8505 Japan.

Country: UNITED STATES

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MEDLINETA: Calcif Tissue Int

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