The hemocyanin of the shamefaced crab Calappa granulata: structural-functional characterization.

The hemocyanin of the shamefaced crab Calappa granulata: structural-functional characterization. Research Abstract Details 

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The hemocyanin of the shamefaced crab Calappa granulata: structural-functional characterization. Abstract Text:

Alessandra Olianas,Maria T Sanna,Irene Messana,Massimo Castagnola,Daniela Masia,Barbara Manconi,Angelo Cau,Bruno Giardina,Mariagiuseppina Pellegrini,

Arthropod hemocyanins (Hcs) transport and store oxygen and are composed of six subunits, or multiples thereof depending on the species. Calappa granulata Hc is found as a mixture of dodecamers (95%) and hexamers (5%). Removal of calcium ions and alkaline pH induce an incomplete partially reversible dissociation of dodecameric Hc. Two-dimensional electrophoretic pattern of dissociated Hc indicated a large heterogeneity in Hc subunit: most differences are likely to be explained by post-translational modifications. Dodecameric Hc showed a large Bohr effect (Deltalog P50/DeltapH = -0.95) and a normal cooperativity (h50 values = 2.7 +/- 0.2) in the presence of 10 mM CaCl2. The hexameric molecule displayed lower Bohr effect and cooperativity than the dodecamer. Lactate effect on the oxygen affinity (Deltalog P50 = 0.55) and the increase of lactate concentrations in animals kept in emersion were related to the increased oxygen requirements that occur during hypoxia in vivo. Calcium affects oxygen affinity only at high concentrations: this Hc appeared to lack the calcium high-affinity binding sites found in other species. The effect of temperature on both oxygen affinity and cooperativity was measured in the absence and presence of 10 mM lactate, allowing calculation of the exothermic contribution of lactate binding (DeltaH = -25 kJ mol(-1)).

The hemocyanin of the shamefaced crab Calappa granulata: structural-functional characterization. Publishing Authors By Initials

A Olianas,MT Sanna,I Messana,M Castagnola,D Masia,B Manconi,A Cau,B Giardina,M Pellegrini,

For similar environment and public health: environment: environment, controlled: temperature research abstracts see: environment and public health: environment: environment, controlled: temperature research

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The hemocyanin of the shamefaced crab Calappa granulata: structural-functional characterization. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 139

Page Numbers: 957-66

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jun

YEAR: 2006

The hemocyanin of the shamefaced crab Calappa granulata: structural-functional characterization. Information

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LANGUAGE: eng

NlmUniqueID: 376600

The hemocyanin of the shamefaced crab Calappa granulata: structural-functional characterization. Keywords Mesh Terms:

KEYWORDS: Temperature

MESH TERMS: metabolism

Chemical & Substance for Abstract: The hemocyanin of the shamefaced crab Calappa granulata: structural-functional characterization. Information

Substance Name: Hemocyanin

Registry Number: 9013-72-3

Grant and Affiliation Information for The hemocyanin of the shamefaced crab Calappa granulata: structural-functional characterization.

AFFILIATION: Dipartimento di Scienze Applicate ai Biosistemi, Università di Cagliari, Cittadella Universitaria, I-09042 Monserrato (CA), Italy.

Country: Japan

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MEDLINETA: J Biochem

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