The G-protein G(o) in mammalian cardiac muscle: localization and coupling to A1 adenosine receptors.

The G-protein G(o) in mammalian cardiac muscle: localization and coupling to A1 adenosine receptors. Research Abstract Details 

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The G-protein G(o) in mammalian cardiac muscle: localization and coupling to A1 adenosine receptors. Abstract Text:

T Asano,H Shinohara,R Morishita,I Norota,K Kato,M Endoh,

To investigate the functional relevance of the G-protein G(o) to adenosine-induced effects in the heart, we studied the localization of G(o) and its interaction with A1 adenosine receptors. Concentrations of G(o) in various mammalian hearts differed markedly between the atrial and the ventricular muscle, as well as among species. In most species examined, the concentration of G(o) was much higher in the atrium than in the ventricle. The highest levels of G(o) in atria and ventricles were found in the ferret heart. An immunohistochemical study of the ferret heart with G(o) alpha-specific antibodies showed that G(o) was localized throughout the membranes of cardiac myocytes, including the intercalated disks. In addition, G(o) was densely distributed in the nerve fibers and Purkinje fibers. Analyses of G(o) alpha subtypes showed that bovine atrium mainly contained G(o)A alpha, while bovine ventricle contained only G(o)B alpha. By contrast, ferret ventricle contained both subtypes of G(o) alpha. To study the coupling of G(o) to A1 adenosine receptors, receptors in ventricular membranes of ferrets, which had been pretreated with pertussis toxin, or purified receptors were reconstituted with purified G(o)A and G(o)B. The reconstitution experiments indicated that both subtypes of G(o) coupled with A1 adenosine receptors. These results suggest that the effect of adenosine is mediated by both G(o)A and G(o)B in membranes of ferret cardiac myocytes.

The G-protein G(o) in mammalian cardiac muscle: localization and coupling to A1 adenosine receptors. Publishing Authors By Initials

T Asano,H Shinohara,R Morishita,I Norota,K Kato,M Endoh,

For similar proteins: membrane proteins: receptors, cell surface: receptors, g-protein-coupled: receptors, purinergic: receptors, purinergic p1 research abstracts see: proteins: membrane proteins: receptors, cell surface: receptors, g-protein-coupled: receptors, purinergic: receptors, purinergic p1 research

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The G-protein G(o) in mammalian cardiac muscle: localization and coupling to A1 adenosine receptors. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 117

Page Numbers: 183-9

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 1995

The G-protein G(o) in mammalian cardiac muscle: localization and coupling to A1 adenosine receptors. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

The G-protein G(o) in mammalian cardiac muscle: localization and coupling to A1 adenosine receptors. Keywords Mesh Terms:

KEYWORDS: Receptors, Purinergic P1

MESH TERMS: metabolism

Chemical & Substance for Abstract: The G-protein G(o) in mammalian cardiac muscle: localization and coupling to A1 adenosine receptors. Information

Substance Name: GTP-Binding Proteins

Registry Number: EC 3.6.1.-

Grant and Affiliation Information for The G-protein G(o) in mammalian cardiac muscle: localization and coupling to A1 adenosine receptors.

AFFILIATION: Department of Biochemistry, Institute for Developmental Research, Aichi.

Country: JAPAN

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MEDLINETA: J Biochem

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