The function of three indigenous plasmids in Mtesorhizobium huakuii 2020 and its symbiotic interaction with Sym pJB5JI of Rhizobium leguminosarum.

The function of three indigenous plasmids in Mtesorhizobium huakuii 2020 and its symbiotic interaction with Sym pJB5JI of Rhizobium leguminosarum. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

The function of three indigenous plasmids in Mtesorhizobium huakuii 2020 and its symbiotic interaction with Sym pJB5JI of Rhizobium leguminosarum. Abstract Text:

Chengyun Yang,Youguo Li,Li Wei,Guojun Cheng,Junchu Zhou,

A Mesorhizobium huakuii strain 2020, isolated from a rice-growing field in southern China, contains three indigenous plasmids named p2020a, p2020b and p2020c, respectively. The plasmids were deleted via Tn5-sacB insertion, and two cured derivatives were obtained. Interestingly, the mutant 2020D29 curing of p2020c could significantly enhance the capacity of symbiotic nitrogen fixation. But the mutant 2020D8 curing of p2020b lost the ability to nodulate Astragalus sinicus. Furthermore, the third plasmid p2020a could be hardly eliminated, suggesting that some house-keeping genes necessary for strain growth located on this plasmid. Then the Sym plasmid pJB5JI of R. leguminosarum bv. viciae was transferred into 2020 and its cured derivatives. The pot plant test showed that the ability of competition and symbiotic nitrogen fixation of transconjugant 2020-137 (pJB5JI) was increased evidently in contrast to 2020. pJB5JI could not restore the ability of 2020D8 to nodulate Astragalus sinicus. 2020D8-8 (pJB5JI) could form ineffective nodules on peas, which implied that the symbiotic plasmid pJB5JI could express its function at the chromosomal background of Mesorhizobium huakuii 2020. The plasmid stability was checked in transconjugants under free-living and during symbiosis. The results indicated that pJB5JI failed to be detected in some nodule isolates. That Km resistance gene could be amplified from all transconjugants and nodule isolates suggested that pJB5JI was fully or partially integrated into the chromosome of recipients.

The function of three indigenous plasmids in Mtesorhizobium huakuii 2020 and its symbiotic interaction with Sym pJB5JI of Rhizobium leguminosarum. Publishing Authors By Initials

C Yang,Y Li,L Wei,G Cheng,J Zhou,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

The function of three indigenous plasmids in Mtesorhizobium huakuii 2020 and its symbiotic interaction with Sym pJB5JI of Rhizobium leguminosarum. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Science in China. Series C, Life sciences / Chines

VOLUME: 51

Page Numbers: 353-61

Journal Abbreviation: Sci. China, C, Life Sci.

ISSN: 1006-9305

DAY: 27

MONTH: 03

YEAR: 2008

The function of three indigenous plasmids in Mtesorhizobium huakuii 2020 and its symbiotic interaction with Sym pJB5JI of Rhizobium leguminosarum. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9611809

The function of three indigenous plasmids in Mtesorhizobium huakuii 2020 and its symbiotic interaction with Sym pJB5JI of Rhizobium leguminosarum. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: The function of three indigenous plasmids in Mtesorhizobium huakuii 2020 and its symbiotic interaction with Sym pJB5JI of Rhizobium leguminosarum. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for The function of three indigenous plasmids in Mtesorhizobium huakuii 2020 and its symbiotic interaction with Sym pJB5JI of Rhizobium leguminosarum.

AFFILIATION: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China.

Country: China

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Sci China C Life Sci

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

The function of three indigenous plasmids in Mtesorhizobium huakuii 2020 and its symbiotic interaction with Sym pJB5JI of Rhizobium leguminosarum Related Publications

• Inhibitors of Na+/H+ and Na+/Ca2+ exchange depress intracellular calcium elevation induced by ischemia/reperfusion in rat cardiac myocytes.
• Inhibitory effect of recombinant FN polypeptide CH50 on the metastasis and tumor growth.
• Quantification of bcr/abl mRNA expression level in leukemia cells by FQ-RT-PCR.
• [An experimental study on the survival of the anterior abdominal skin flap after VEGF cDNA administration]
• [An inquiry into blood loss in laparoscopic liver resections]
• [Association studies of dopamine D4 receptor gene and dopamine transporter gene polymorphisms in Han Chinese patients with attention deficit hyperactivity disorder]
• [Beneficial effect of thyrotropin-releasing hormone in combination with HSD on hemorrhagic shock with pulmonary edema at high altitude in the rat]
• [Changes in platelet membrane glycoprotein levels in patients with cardiopulmonary resuscitation]
• [Clinical analysis of 13 infected total knee replacements]
• [Detection of epileptic waves in EEG based on wavelet transform]
• [Effects of Alexandrium tamarense and Prorocentrum donghaiense on rotifer Brachionus plicatilis population]
• [Effects of the novel gene, LAPTM4B, highly expression in hepatocellular carcinoma on cell proliferation and tumorigenesis of NIH3T3 cells]
• [Experimental study of K562 cell apoptosis induced by harringtonine]
• [Expression of otsA gene in tobacco and improvement stress tolerance]
• [Hemodynamic and pathologic changes in open abdominal wound after seawater immersion in dog]
• [High efficient expression of recombinant human platelet factor IV in pichia pastoris]
• [Identification of radix Rubiae and the root of Humulus scardens (Lour.) Merr.]
• [Incompatibility among indigenous plasmids of Sinorhizobium fredii strains and its application for plasmid curing]
• [Nutrition support of severe illness patients of general surgery during perioperation period]
• [Preliminary analysis of the characteristics of red tide areas in Changjiang River estuary and its adjacent sea]
• [Relationship between tissue inhibitors of metalloproteinase and metastasis and prognosis in breast cancer]
• [Repairing large alar defects by using a retro-grade auricular flap]
• [Research advance in effects of harmful algal bloom species on zooplankton]
• [Scoring protocol for quantitative diagnosis of gastrointestinal dysfunction and failure in severe cases]
• [Study on new techniques for genetic diagnosis of deletional alpha-thalassemia]
• [The influence of intrahepatic transplantation of hepatocytes and insular cells on liver cirrhosis]
• [The relationship of facet orientation to intervertebral disc protrusion and lateral recess stenosis in lower lumbar spine]
• [Transplantation of bone marrow cells upregulated vascular endothelial growth factor and its receptor and improved ischemic myocardial function]
• [Ultrasound surveillance of cervical lymph node metastasis in thoracic esophageal carcinoma]
• [Using scar tissue flap to repair secondary nasal deformities of unilateral cleft lip]