[The function of 5'flanking region of mouse neuronal CDC2-like kinase regulatory subunit p35]

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[The function of 5'flanking region of mouse neuronal CDC2-like kinase regulatory subunit p35] Abstract Text:

H Zhu,C Zhou,J Fu,

OBJECTIVE: To clarify the possible mechanisms which control p35(nck5a) neural-specific expression. METHODS: Thirteen luciferase expression vectors which contain different parts of 5'flanking sequence were constructed. Transient expression assay, DNase 1 hypersensitive site(DHSS) assay and primer extension assay were made to reveal the possible mechanisms of p35(nck5a) expression control. RESULTS: It was found that 5'flanking sequence of p35(nck5a) contained 5455bp and there were a few possible promoters in the fragment. The result of transient expression assay in primary cerebrum cortex cell of rat (Neuron) and established non-neuron cell such as HeLa showed the loss of neural-specific expression of all the luciferase expression vectors. The result of DNase 1 hypersensitive site assay revealed a DHSS located at the -400bp upstream of p35(nck5a) in brain but not in liver. The primer extension assay found the putative transcription initiation site near the position of DHSS. CONCLUSION: The results above suggest that there is no cis-element to control the neural-specific expression in the 5'flanking sequence, and the control on the chromosome level may be critical to the neural-specific expression of p35(nck5a).

[The function of 5'flanking region of mouse neuronal CDC2-like kinase regulatory subunit p35] Publishing Authors By Initials

H Zhu,C Zhou,J Fu,

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[The function of 5'flanking region of mouse neuronal CDC2-like kinase regulatory subunit p35] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Zhonghua yi xue yi chuan xue za zhi = Zhonghua yix

VOLUME: 18

Page Numbers: 251-4

Journal Abbreviation: Zhonghua Yi Xue Yi Chuan Xue Z

ISSN: 1003-9406

DAY: 2

MONTH: Aug

YEAR: 2001

[The function of 5'flanking region of mouse neuronal CDC2-like kinase regulatory subunit p35] Information

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LANGUAGE: chi

NlmUniqueID: 9425197

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AFFILIATION: Department of Medical Genetics, the Second Military Medical University, Shanghai 200433 P. R. China.

Country: China

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MEDLINETA: Zhonghua Yi Xue Yi Chuan Xue Z

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