The expression pattern of the subunit of chaperonin containing T-complex polypeptide 1 and its substrate, alpha-smooth muscle actin, during corneal wound healing.

The expression pattern of the subunit of chaperonin containing T-complex polypeptide 1 and its substrate, alpha-smooth muscle actin, during corneal wound healing. Research Abstract Details 

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The expression pattern of the subunit of chaperonin containing T-complex polypeptide 1 and its substrate, alpha-smooth muscle actin, during corneal wound healing. Abstract Text:

PURPOSE: This study was designed to demonstrate the expression of the chaperonin containing T-complex polypeptide 1 (CCT) and alpha-smooth muscle actin (alpha-SMA), in normal corneas and corneas treated with ultraviolet radiation (UVR). The wound model chosen is previously characterized, the injury is mild and the cornea heals to transparency. METHODS: Rabbit corneas were exposed to UVR at the dose producing keratitis. The corneas were allowed to heal for up to 5 days and the paraffin-embedded tissue specimens were double stained and examined morphologically and immunohistochemically. Expression of CCT and alpha-SMA genes was investigated by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: There was a front of repopulating keratocytes that showed positive staining for alpha-SMA after 3 days. The alpha-SMA mRNA was already strongly expressed after 1 day, whereas the expression level of CCT was increased after 2 days. After 5 days the levels were decreased. By this time the stroma was partly repopulated by keratocytes. CONCLUSION: In a mild wound, the expression of alpha-SMA mRNA is followed by expression of mRNA of at least one subunit of the complex folding alpha-SMA. At protein level, alpha-SMA is detected in the front line of repopulating keratocytes. Expression levels for both mRNAs decline as the stroma repopulation process progresses.

The expression pattern of the subunit of chaperonin containing T-complex polypeptide 1 and its substrate, alpha-smooth muscle actin, during corneal wound healing. Publishing Authors By Initials

For similar biological phenomena, cell phenomena, and immunity: biological phenomena: regeneration: wound healing research abstracts see: biological phenomena, cell phenomena, and immunity: biological phenomena: regeneration: wound healing research

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The expression pattern of the subunit of chaperonin containing T-complex polypeptide 1 and its substrate, alpha-smooth muscle actin, during corneal wound healing. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Acta ophthalmologica Scandinavica

VOLUME: 83

Page Numbers: 543-8

Journal Abbreviation: Acta Ophthalmol Scand

ISSN: 1395-3907

DAY: 17

MONTH: Oct

YEAR: 2005

The expression pattern of the subunit of chaperonin containing T-complex polypeptide 1 and its substrate, alpha-smooth muscle actin, during corneal wound healing. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9507578

The expression pattern of the subunit of chaperonin containing T-complex polypeptide 1 and its substrate, alpha-smooth muscle actin, during corneal wound healing. Keywords Mesh Terms:

KEYWORDS: Wound Healing

MESH TERMS: metabolism

Chemical & Substance for Abstract: The expression pattern of the subunit of chaperonin containing T-complex polypeptide 1 and its substrate, alpha-smooth muscle actin, during corneal wound healing. Information

Substance Name: t-complex polypeptide-1

Registry Number: 0

Grant and Affiliation Information for The expression pattern of the subunit of chaperonin containing T-complex polypeptide 1 and its substrate, alpha-smooth muscle actin, during corneal wound healing.

AFFILIATION: Department of Ophthalmology, Linköping University Hospital, Linköping, Sweden. mark@ste.ki.se

Country: Denmark

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MEDLINETA: Acta Ophthalmol Scand

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