[The experimental study of transfected sFlt-Ig gene on K562 leukemia cell growth in nude mice]

[The experimental study of transfected sFlt-Ig gene on K562 leukemia cell growth in nude mice] Research Abstract Details 

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[The experimental study of transfected sFlt-Ig gene on K562 leukemia cell growth in nude mice] Abstract Text:

J Zhao,J Chu,S Wang,S Xu,

OBJECTIVE: To observe the influence of transfected soluble vascular endothelial growth factor (VEGF) receptor (sFlt-1) gene on K562 leukemia cell growth in vivo. METHODS: (1) The binding region of VEGF receptor (Flt-1) ligand was combined with fragment of IgH stable region to construct Flt-Ig fusion gene and insert into pcDNA3 vector. (2) By using electroporation, the pcDNA3/Flt-Ig was transfected into K562 leukemia cells, and selected by G418. Flt-Ig mRNA expression was detected by RT-PCR. (3) The transfected pcDNA3/Flt-Ig and pcDNA3-Ig K562 cells were respectively transplanted into nude mice and the tumor volume was dynamically measured. RESULTS: Five subclones of K562 cells with high expression of Flt-Ig gene have been established, one of them was transplanted into 6 nude mice. The tumor volume of experimental mice was obviously smaller than that of control mice, about one half of the control group (P < 0.05). CONCLUSION: The growth of transfected pcDNA3/Flt-Ig K562 cells was significantly inhibited. It is possible that soluble Flt-Ig protein secreted from K562/Flt-Ig cells neutralized VEGF produced from tumor cells, therefore inhibited the tumor angiogenesis.

[The experimental study of transfected sFlt-Ig gene on K562 leukemia cell growth in nude mice] Publishing Authors By Initials

J Zhao,J Chu,S Wang,S Xu,

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[The experimental study of transfected sFlt-Ig gene on K562 leukemia cell growth in nude mice] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Zhonghua xue ye xue za zhi = Zhonghua xueyexue zaz

VOLUME: 22

Page Numbers: 229-31

Journal Abbreviation: Zhonghua Xue Ye Xue Za Zhi

ISSN: 0253-2727

DAY: 5

MONTH: May

YEAR: 2001

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LANGUAGE: chi

NlmUniqueID: 8212398

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AFFILIATION: Institute of Hematology, CAMS & PUMC, State key Lab. of Experimental Hematology, Tianjin 300020, China.

Country: China

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MEDLINETA: Zhonghua Xue Ye Xue Za Zhi

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