The effects of simulated microgravity on cultured chicken embryonic chondrocytes.

The effects of simulated microgravity on cultured chicken embryonic chondrocytes. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

The effects of simulated microgravity on cultured chicken embryonic chondrocytes. Abstract Text:

X Zhang,X B Li,S Z Yang,S G Li,P D Jiang,Z H Lin,

Using the cultured chicken embryonic chondrocytes as a model, the effects of simulated microgravity on the microtubular system of the cellular skeleton, extracellular matrix, alkaline phosphatase activity, intracellular free calcium concentration and mitochondrial ATP synthase activity with its oligomycin inhibition rate were studied with a clinostat. The microtubular content was measured by a flow cytometer. The decrease of microtubular content showed the impairment of the cellular skeleton system. Observation on the extracellular matrix by the scanning electron microscopy showed that it decreased significantly after rotating, and the fibers in the extracellular matrix were more tiny and disorderly than that of the control group. It can be concluded that the simulated microgravity can affect the secreting and assembly of the extracellular matrix. In contrast to the control, there was a time course decrease in alkaline phosphatase activity of chondrocytes, a marker of matrix mineralization. Meanwhile a significant drop in the intracellular calcium concentration happened at the beginning of rotation. These results indicate that simulated microgravity can suppress matrix calcification of cultured chondrocytes, and intracellular free calcium may be involved in the regulation of matrix calcification as the second signal transmitter. No significant changes happened in the mitochondrial ATP synthase activity and its oligomycin inhibition rate. Perhaps the energy metabolism wasn't affected by the simulated microgravity. The possible mechanisms about them were discussed.

The effects of simulated microgravity on cultured chicken embryonic chondrocytes. Publishing Authors By Initials

X Zhang,XB Li,SZ Yang,SG Li,PD Jiang,ZH Lin,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

The effects of simulated microgravity on cultured chicken embryonic chondrocytes. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Advances in space research : the official journal

VOLUME: 32

Page Numbers: 1577-83

Journal Abbreviation: Adv Space Res

ISSN: 0273-1177

DAY: 5

MONTH: 03

YEAR: 2003

The effects of simulated microgravity on cultured chicken embryonic chondrocytes. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9878935

The effects of simulated microgravity on cultured chicken embryonic chondrocytes. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: The effects of simulated microgravity on cultured chicken embryonic chondrocytes. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for The effects of simulated microgravity on cultured chicken embryonic chondrocytes.

AFFILIATION: Institute of Biophysics, Chinese Academy of Science, Beijing, China.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Adv Space Res

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

The effects of simulated microgravity on cultured chicken embryonic chondrocytes Related Publications

• A Drosophila doublesex-related gene, terra, is involved in somitogenesis in vertebrates.
• Antropyloroduodenal motility after resection of esophageal cancer.
• Family practice graduate preparedness in the six ACGME competency areas: prequel.
• Identification of MUC5B mucin gene in human middle ear with chronic otitis media.
• Multiple suppressor gene p16 of human brain gliomas.
• Requirement of a novel gene, Xin, in cardiac morphogenesis.
• Signal irreproducibility in high-field solution magnetic resonance experiments caused by spin turbulence.
• The effects of simulated microgravity on cultured chicken embryonic chondrocytes.
• Vitamin E succinate potentiates the inhibitory effect of prostaglandins on oral squamous carcinoma cell proliferation.
• [A novel bcl-2 antisense oligodeoxynucleotide F951 increases sensitivity of HL-60 cells to Ara-C]
• [Analysis of essential oil from Artemisia annul L. by extraction of different methods]
• [Arsenic trioxide induced p15INK4B gene expression in myelodysplastic syndrome cell line MUTZ-1]
• [Association between gene mutation of cytochrome P450 1A1 in exon 7 A4889G locus and susceptibility to endometriosis]
• [Cephalometric comparison of soft-tissue morphology between extraction and non-extraction orthodontic treatment in borderline cases.]
• [Clinical evaluation of two tooth preparation methods of metal-porcelain restoration]
• [Clinical study on changes of heme oxygenase-1 expression in patients with acute myocardial infarction]
• [Cloning and expression of heavy- and light-chain variable region genes of monoclonal antibody specific for human fibrin]
• [Diagnosis of lesions in the major abdominal blood vessels by three-dimensional dynamic contrast-enhanced magnetic resonance angiography]
• [Effect of cyclosporin A on intracellular accumulation and efflux of drug in K562/DOX cells]
• [Effects of catecholamines on human preadipocyte proliferation and differentiation]
• [Establishment and characterization of a neural stem cell line (RNSC-FMU 1) from rat brain]
• [Expression and significance of nitric oxide synthase in human hepatocellular carcinoma]
• [Mechanism of injury by active oxygen radical to hemoglobin]
• [Recent researches on fungal hydrophobins]
• [Stability of molar relationship after orthodontic treatment in Class II division 1 malocclusions treated by extraction]
• [Studies on purgative activity of rhubarb extracts by different technologies]
• [Study on induction of leukemic cell apoptosis by antisense oligodeoxynucleotide of human telomerase RNA]
• [Susceptibility to endometriosis in women of Han Nationality in Guangdong Province associated with Msp I polymorphisms of cytochrome P450 1A1 gene]
• [The endocrinological function of ghrelin]
• [Three-dimensional measurement of tooth inclination in cases with successful treatment]