The effect of various stain carriers on the quality and quantity of DNA extracted from dried bloodstains.

The effect of various stain carriers on the quality and quantity of DNA extracted from dried bloodstains. Research Abstract Details 

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The effect of various stain carriers on the quality and quantity of DNA extracted from dried bloodstains. Abstract Text:

M Prinz,G Berghaus,

Bloodstains were made with 200 microliters blood on each of 11 different common substrates to examine the effect of the stain carrier on the amount and quality of DNA recoverable. High-molecular-weight DNA was extracted from all samples after 2 days. The yield of DNA from each sample varied considerably, not only between the different stain carriers but also within a given category. With a DNA yield of up to 10 micrograms, paper, glass, nylon, wood, smooth leather and wool gave the best results, followed by blue denim and wallpaper (up to 6 micrograms), cotton fabric and carpeting (up to 4 micrograms) and suede (up to 2 micrograms). For several stain carriers the DNA-containing solution was contaminated by chemical substances, which in the case of the blue denim, suede, and carpet samples inhibited the digestion of the DNA with restriction enzymes and prevented DNA typing. The different textures of the stain carriers tested and (as for varying yields on the same carrier) the differing degree of loss of DNA during extraction and the physiological variation in the number of leukocytes in human blood are discussed as possible reasons for the wide range of variation in the amounts of DNA it was possible to extract.

The effect of various stain carriers on the quality and quantity of DNA extracted from dried bloodstains. Publishing Authors By Initials

M Prinz,G Berghaus,

For similar diagnosis: laboratory techniques and procedures: sex determination (analysis) research abstracts see: diagnosis: laboratory techniques and procedures: sex determination (analysis) research

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The effect of various stain carriers on the quality and quantity of DNA extracted from dried bloodstains. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Zeitschrift für Rechtsmedizin. Journal of legal me

VOLUME: 103

Page Numbers: 191-7

Journal Abbreviation: Z. Rechtsmed.

ISSN: 0044-3433

DAY: 20

MONTH: 02

YEAR: 1990

The effect of various stain carriers on the quality and quantity of DNA extracted from dried bloodstains. Information

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LANGUAGE: eng

NlmUniqueID: 247437

The effect of various stain carriers on the quality and quantity of DNA extracted from dried bloodstains. Keywords Mesh Terms:

KEYWORDS: Sex Determination (Analysis)

MESH TERMS: genetics

Chemical & Substance for Abstract: The effect of various stain carriers on the quality and quantity of DNA extracted from dried bloodstains. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for The effect of various stain carriers on the quality and quantity of DNA extracted from dried bloodstains.

AFFILIATION: Institut für Rechtsmedizin, Universität Köln, Federal Republic of Germany.

Country: GERMANY, WEST

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MEDLINETA: Z Rechtsmed

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