The effect of substitution of the N-acetyl groups of N-acetylgalactosamine residues in chondroitin sulfate on its degradation by chondroitinase ABC.

The effect of substitution of the N-acetyl groups of N-acetylgalactosamine residues in chondroitin sulfate on its degradation by chondroitinase ABC. Research Abstract Details 

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The effect of substitution of the N-acetyl groups of N-acetylgalactosamine residues in chondroitin sulfate on its degradation by chondroitinase ABC. Abstract Text:

Subbarao V Madhunapantula,Rajeshwara N Achur,Veer P Bhavanandan,D Channe Gowda,Subbarao V Madhunapantula,Rajeshwara N Achur,Veer P Bhavanandan,D Channe Gowda,

Chondroitinase ABC is a lyase that degrades chondroitin sulfate, dermatan sulfate and hyaluronic acid into disaccharides. The purpose of this study was to determine the ability of chondroitinase ABC to degrade chondroitin sulfate in which the N-acetyl groups are substituted with different acyl groups. The bovine tracheal chondroitin sulfate A (bCSA) was N-deacetylated by hydrazinolysis, and the free amino groups derivatized into N-formyl, N-propionyl, N-butyryl, N-hexanoyl or N-benzoyl amides. Treatment of the N-acyl or N-benzoyl derivatives of bCSA with chondroitinase ABC and analysis of the products showed that the N-formyl, N-hexanoyl and N-benzoyl derivatives are completely resistant to the enzyme. In contrast, the N-propionyl or N-butyryl derivatives were degraded into disaccharides with slower kinetics compared to that of unmodified bCSA. The rate of degradation of bCSA derivatives by the enzyme was found to be in the order of N-acetyl>N-propionyl>>N-butyryl bCSA. These results have important implications for understanding the interaction of N-acetyl groups of glycosaminoglycans with chondroitinase ABC.

The effect of substitution of the N-acetyl groups of N-acetylgalactosamine residues in chondroitin sulfate on its degradation by chondroitinase ABC. Publishing Authors By Initials

SV Madhunapantula,RN Achur,VP Bhavanandan,DC Gowda,SV Madhunapantula,RN Achur,VP Bhavanandan,DC Gowda,

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The effect of substitution of the N-acetyl groups of N-acetylgalactosamine residues in chondroitin sulfate on its degradation by chondroitinase ABC. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Glycoconjugate journal

VOLUME: 24

Page Numbers: 465-73

Journal Abbreviation: Glycoconj. J.

ISSN: 0282-0080

DAY: 29

MONTH: 05

YEAR: 2007

The effect of substitution of the N-acetyl groups of N-acetylgalactosamine residues in chondroitin sulfate on its degradation by chondroitinase ABC. Information

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LANGUAGE: eng

NlmUniqueID: 8603310

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Grant and Affiliation Information for The effect of substitution of the N-acetyl groups of N-acetylgalactosamine residues in chondroitin sulfate on its degradation by chondroitinase ABC.

AFFILIATION: Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, 500 University Drive, Hershey, PA 17033, USA.

Country: United States

AGENCY: United States NIAID

GRANT: AI45086

ACRONYM: AI

MEDLINETA: Glycoconj J

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