[The effect of monovalent cations on the activity of site-specific endonuclease PaeII]

[The effect of monovalent cations on the activity of site-specific endonuclease PaeII] Research Abstract Details 

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[The effect of monovalent cations on the activity of site-specific endonuclease PaeII] Abstract Text:

Activity of restrictase Pae II, contrary to known restriction enzymes of the II class (except of true isoshizomere Sma), depended absolutely on monovalent cations. This pattern is untypical for restrictases of the II class. At the same time, restrictase Pae II was able to hydrolyze DNA as a substrate in absence of exogenous Mg2+, in the incubation mixture contained cations K+, Rb+, Cs+ and NH4+ but not Na+ or Li+. Mg2+ was found to activate the enzyme in presence of monovalent cations. Basing on the protective effect on K+ against inactivation of restrictase Pae II by means of thiol-affecting reagents and high temperature as well as on stabilization of the enzyme by KCl during storage, monovalent cations appear to participate in formation of protein molecule structure, which is optimal for catalytic effect and resistant to inactivation.

[The effect of monovalent cations on the activity of site-specific endonuclease PaeII] Publishing Authors By Initials

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PUBMED ID PMID:

MEDLINE DATE: 1990 Jan-Feb

[The effect of monovalent cations on the activity of site-specific endonuclease PaeII] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Voprosy medit?sinsko? khimii

VOLUME: 36

Page Numbers: 65-7

Journal Abbreviation: Vopr. Med. Khim.

ISSN: 0042-8809

DAY: 29

MONTH: 08

YEAR: 2008

[The effect of monovalent cations on the activity of site-specific endonuclease PaeII] Information

Number of References:

LANGUAGE: rus

NlmUniqueID: 416601

[The effect of monovalent cations on the activity of site-specific endonuclease PaeII] Keywords Mesh Terms:

KEYWORDS: Pseudomonas aeruginosa

MESH TERMS: enzymology

Chemical & Substance for Abstract: [The effect of monovalent cations on the activity of site-specific endonuclease PaeII] Information

Substance Name: Deoxyribonucleases, Type II Site-Specifi

Registry Number: EC 3.1.21.4

Grant and Affiliation Information for [The effect of monovalent cations on the activity of site-specific endonuclease PaeII]

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Country: USSR

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MEDLINETA: Vopr Med Khim

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