The effect of insulin on expression of genes and biochemical pathways in human skeletal muscle.

The effect of insulin on expression of genes and biochemical pathways in human skeletal muscle. Research Abstract Details 

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The effect of insulin on expression of genes and biochemical pathways in human skeletal muscle. Abstract Text:

Xuxia Wu,Jelai Wang,Xiangqin Cui,Lidia Maianu,Brian Rhees,James Rosinski,W Venus So,Steven M Willi,Michael V Osier,Helliner S Hill,Grier P Page,David B Allison,Mitchell Martin,W Timothy Garvey,

To study the insulin effects on gene expression in skeletal muscle, muscle biopsies were obtained from 20 insulin sensitive individuals before and after euglycemic hyperinsulinemic clamps. Using microarray analysis, we identified 779 insulin-responsive genes. Particularly noteworthy were effects on 70 transcription factors, and an extensive influence on genes involved in both protein synthesis and degradation. The genetic program in skeletal muscle also included effects on signal transduction, vesicular traffic and cytoskeletal function, and fuel metabolic pathways. Unexpected observations were the pervasive effects of insulin on genes involved in interacting pathways for polyamine and S-adenoslymethionine metabolism and genes involved in muscle development. We further confirmed that four insulin-responsive genes, RRAD, IGFBP5, INSIG1, and NGFI-B (NR4A1), were significantly up-regulated by insulin in cultured L6 skeletal muscle cells. Interestingly, insulin caused an accumulation of NGFI-B (NR4A1) protein in the nucleus where it functions as a transcription factor, without translocation to the cytoplasm to promote apoptosis. The role of NGFI-B (NR4A1) as a new potential mediator of insulin action highlights the need for greater understanding of nuclear transcription factors in insulin action.

The effect of insulin on expression of genes and biochemical pathways in human skeletal muscle. Publishing Authors By Initials

X Wu,J Wang,X Cui,L Maianu,B Rhees,J Rosinski,WV So,SM Willi,MV Osier,HS Hill,GP Page,DB Allison,M Martin,WT Garvey,

For similar proteins: transcription factors research abstracts see: proteins: transcription factors research

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The effect of insulin on expression of genes and biochemical pathways in human skeletal muscle. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Endocrine

VOLUME: 31

Page Numbers: 5-17

Journal Abbreviation: Endocrine

ISSN: 0969-711X

DAY: 3

MONTH: Feb

YEAR: 2007

The effect of insulin on expression of genes and biochemical pathways in human skeletal muscle. Information

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LANGUAGE: eng

NlmUniqueID: 9434444

The effect of insulin on expression of genes and biochemical pathways in human skeletal muscle. Keywords Mesh Terms:

KEYWORDS: Transcription Factors

MESH TERMS: physiology

Chemical & Substance for Abstract: The effect of insulin on expression of genes and biochemical pathways in human skeletal muscle. Information

Substance Name: orphan nuclear receptor NGFI-B

Registry Number: 121479-42-3

Grant and Affiliation Information for The effect of insulin on expression of genes and biochemical pathways in human skeletal muscle.

AFFILIATION: Department of Nutrition Sciences, University of Alabama at Birmingham, 1675 University Boulevard, Birmingham, AL 35294-3360, USA. xuxiawu@uab.edu

Country: United States

AGENCY: United States NIDDK

GRANT: P30-DK56336

ACRONYM: DK

MEDLINETA: Endocrine

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