The effect of hyaluronic acid incorporation on fibroblast spreading and proliferation within PEG-diacrylate based semi-interpenetrating networks.

The effect of hyaluronic acid incorporation on fibroblast spreading and proliferation within PEG-diacrylate based semi-interpenetrating networks. Research Abstract Details 

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The effect of hyaluronic acid incorporation on fibroblast spreading and proliferation within PEG-diacrylate based semi-interpenetrating networks. Abstract Text:

Jaishankar K Kutty,Eunhee Cho,Jeoung Soo Lee,Naren R Vyavahare,Ken Webb,Jaishankar K Kutty,Eunhee Cho,Jeoung Soo Lee,Naren R Vyavahare,Ken Webb,

The nanometer-scale mesh size of many synthetic crosslinked hydrogel networks restricts encapsulated cells to a rounded morphology that can inhibit cellular processes such as proliferation and migration that are essential for the early stages of remodeling and tissue formation. The objective of these studies was to investigate an approach for accelerating cellular remodeling based on the creation of semi-interpenetrating networks (IPNs) composed of hydrolytically degradable poly(ethylene glycol) (PEG) diacrylate macromers and native, enzymatically degradable extracellular matrix (ECM) components (collagen, gelatin and hyaluronic acid (HA)). Among the three ECM components investigated, addition of HA at concentrations of 0.12% w/v and greater supported fibroblast spreading throughout the three-dimensional network and significantly increased proliferation relative to control hydrogels without HA. Incorporation of HA resulted in relatively small changes in hydrogel physical/chemical properties such as swelling, degradation rate, and elastic modulus. Fibroblast spreading was eliminated by the addition of hyaluronidase inhibitors, demonstrating that cell-mediated enzymatic degradation of HA is a necessary mechanism responsible for the observed increases in fibroblast activity. By accelerating early cellular remodeling and growth, these semi-IPNs may be useful vehicles for cell transplantation in a variety of tissue engineering applications.

The effect of hyaluronic acid incorporation on fibroblast spreading and proliferation within PEG-diacrylate based semi-interpenetrating networks. Publishing Authors By Initials

JK Kutty,E Cho,J Soo Lee,NR Vyavahare,K Webb,JK Kutty,E Cho,J Soo Lee,NR Vyavahare,K Webb,

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The effect of hyaluronic acid incorporation on fibroblast spreading and proliferation within PEG-diacrylate based semi-interpenetrating networks. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biomaterials

VOLUME: 28

Page Numbers: 4928-38

Journal Abbreviation: Biomaterials

ISSN: 0142-9612

DAY: 27

MONTH: 08

YEAR: 2007

The effect of hyaluronic acid incorporation on fibroblast spreading and proliferation within PEG-diacrylate based semi-interpenetrating networks. Information

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LANGUAGE: eng

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Grant and Affiliation Information for The effect of hyaluronic acid incorporation on fibroblast spreading and proliferation within PEG-diacrylate based semi-interpenetrating networks.

AFFILIATION: Micro-Environmental Engineering Laboratory, Department of Bioengineering, Clemson University, 501 Rhodes Research Center, Clemson, SC 29634, USA.

Country: England

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MEDLINETA: Biomaterials

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