The effect of esterases on 17alpha-hydroxyprogesterone caproate.

The effect of esterases on 17alpha-hydroxyprogesterone caproate. Research Abstract Details 

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The effect of esterases on 17alpha-hydroxyprogesterone caproate. Abstract Text:

Ru Yan,Valentina Fokina,Gary D V Hankins,Mahmoud S Ahmed,Tatiana N Nanovskaya,Ru Yan,Valentina Fokina,Gary D V Hankins,Mahmoud S Ahmed,Tatiana N Nanovskaya,Ru Yan,Valentina Fokina,Gary D V Hankins,Mahmoud S Ahmed,Tatiana N Nanovskaya,

OBJECTIVE: The aim of this investigation is to determine whether 17alpha-hydroxyprogesterone caproate is hydrolyzed, in vitro, to 17alpha-hydroxyprogesterone and caproate. STUDY DESIGN: The in vitro hydrolysis of dual radioactively labeled 17alpha-hydroxy-[(3)H] progesterone [(14)C] caproate by human plasma, hepatic and placental S9 fractions as well as recombinant esterases was investigated. The formation of [(3)H]-17alpha-hydroxyprogesterone and [(14)C]-caproate were determined with the use of high-performance liquid chromatography equipped with an online radioactivity detector. The presence and activity of carboxylesterase and butyrylcholinesterase in the human-derived preparations was confirmed by the hydrolysis of their prototypic substrates p-nitrophenyl acetate, p-nitrophenyl butyrate, and butyrylthiocholine, respectively. RESULTS: The aforementioned human-derived preparations hydrolyzed p-nitrophenyl acetate, p-nitrophenyl butyrate, and butyrylthiocholine. However, when 17alpha-hydroxyprogesterone caproate was incubated with the human-derived preparations under identical experimental conditions neither [(3)H]-17alpha-hydroxyprogesterone nor [(14)C]-caproate was detected. CONCLUSION: 17alpha-hydroxyprogesterone caproate is not hydrolyzed in vitro by the esterase enzymes present in human plasma, liver, preterm, or term placenta.

The effect of esterases on 17alpha-hydroxyprogesterone caproate. Publishing Authors By Initials

R Yan,V Fokina,GD Hankins,MS Ahmed,TN Nanovskaya,R Yan,V Fokina,GD Hankins,MS Ahmed,TN Nanovskaya,R Yan,V Fokina,GD Hankins,MS Ahmed,TN Nanovskaya,

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The effect of esterases on 17alpha-hydroxyprogesterone caproate. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: American journal of obstetrics and gynecology

VOLUME: 198

Page Numbers: 229.e1-5

Journal Abbreviation: Am. J. Obstet. Gynecol.

ISSN: 1097-6868

DAY: 22

MONTH: 10

YEAR: 2007

The effect of esterases on 17alpha-hydroxyprogesterone caproate. Information

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LANGUAGE: eng

NlmUniqueID: 370476

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Grant and Affiliation Information for The effect of esterases on 17alpha-hydroxyprogesterone caproate.

AFFILIATION: Department of Obstetrics & Gynecology, University of Texas Medical Branch, Galveston, TX.

Country: United States

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MEDLINETA: Am J Obstet Gynecol

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