The effect of electrical fields on gene and protein expression in human osteoarthritic cartilage explants.

The effect of electrical fields on gene and protein expression in human osteoarthritic cartilage explants. Research Abstract Details 

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The effect of electrical fields on gene and protein expression in human osteoarthritic cartilage explants. Abstract Text:

Carl T Brighton,Wei Wang,Charles C Clark,

BACKGROUND: The destruction of cartilage in patients with osteoarthritis is a consequence of an imbalance between matrix synthesis and degradation. The purpose of the present study was to determine the effects of electrical stimulation on these processes in full-thickness osteoarthritic adult human articular cartilage explants. METHODS: Full-thickness articular cartilage explants from osteoarthritic adult human knee joints were cultured in the absence or presence of interleukin-1beta (IL-1beta) and in the absence or presence of a specifically defined capacitively coupled electrical signal for seven or fourteen days. Total collagen and proteoglycan production were assessed by means of hydroxyproline and hexosamine analyses, respectively. Quantitative real-time polymerase chain reaction assays were used to measure mRNA expression levels of aggrecan, type-II collagen, collagenase-1 (MMP-1), collagenase-3 (MMP-13), stromelysin-1 (MMP-3), aggrecanase-1 (ADAM-TS4), and aggrecanase-2 (ADAM-TS5). RESULTS: Electrical stimulation of cultured explants for seven or fourteen days resulted in significant increases (p < 0.007) in proteoglycan and collagen production and a highly significant upregulation (p

The effect of electrical fields on gene and protein expression in human osteoarthritic cartilage explants. Publishing Authors By Initials

CT Brighton,W Wang,CC Clark,

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The effect of electrical fields on gene and protein expression in human osteoarthritic cartilage explants. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of bone and joint surgery. American vo

VOLUME: 90

Page Numbers: 833-48

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ISSN: 1535-1386

DAY: 2

MONTH: Apr

YEAR: 2008

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LANGUAGE: eng

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AFFILIATION: McKay Laboratory of Orthopaedic Surgery Research, University of Pennsylvania School of Medicine, 424 Stemmler Hall, Philadelphia, PA 19104-6081, USA. ctb@mail.med.upenn.edu

Country: United States

AGENCY: United States NIAMS

GRANT: AR050950

ACRONYM: AR

MEDLINETA: J Bone Joint Surg Am

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